Association Of C3 And DAF Genetic Variant With The Risk Of Gastric Cancer

Objective This study aims to investigate the association of genetic variants in complement 3(C3) and decay-accelerating factor(DAF) genes with the risk of gastric cancer.Methods This case-control study included 500 gastric cancer and 500 cancer-free controls. All subjects were unrelated with Han Chinese from Tangshan city and its surrounding region. Patients were recruited between Jan 2008 and Dec 2013 from Tangshan Gongren Hospital and Tangshan Renmin Hospital in China. Based on the Chinese population data from Hap Map database, we used Haploview 4.2 program to select candidate tag SNPs. Genotyping was performed using Sequenom Mass Array. The differences in distribution of selected characteristics between cases and controls were tested by χ2 test. The associations between DAF, C3 genotypes and gastric cancer risk were estimated by odds ratios(ORs) and their 95% confidence intervals(95%CI).Results Using Haploview Program, we selected 12 tag SNP of C3 and 2 tag SNP(rs220199, rs2230205, rs2241393, rs2250656, rs2277984, rs2287846, rs3745568, rs432001, rs11569523, rs379527, rs2230204, rs433594) of DAF(rs10746463, rs2564978). The proportion of DAF rs10746463 GG, GA and AA genotype was 27.0%, 52.2%, 20.8% and 34.0%, 48.4%, 17.6% in cases and controls, respectively. Logistic regression analysis revealed that the carriers with DAF rs10746463 AA genotype had a significantly increased risk for developing gastric cancer(OR=1.46, 95% CI=1.01-2.10) when compared with GG genotype. When stratified by smoking status, we found that the risk of gastric cancer was associated with rs10746463 GA or AA genotype carriers among smoker with OR(95%CI) of 1.64(1.06-2.54), but not among non-smoker(OR=1.37, 95%CI= 0.97-1.94). Our data didn’t show any significant association of DAF rs2564978 and C3 SNPs polymorphism with the gastric cancer susceptibility.Conclusion DAF rs10746463 polymorphism effects on the risk of developing gastric cancer in North Chinese population.