| Background:Chronic renal failure(CRF) is a clinical syndrome with progressive damage in renal units, mainly as azotemia,water and electrolyte disorders, acid-base imbalance, metabolites retention and generalized symptoms,is the final destination of a variety of kidney disease sustain development. According to relevant statistics, the global end-stage renal failure on dialysis sustain life as many as 360 million.The variety of kidney patients is more than 4000 million people in China,while the annual number of new patients with CRF occurred at around 10-30 million.Some predict,in the next decade the CRF treatment costs at least 1.1 trillion,such a large cost very embarrassing for the government.Chinese medicine compound prevention and treatment CRF curative effect.Fushengongdecoction is by the master of the first national physician, national famous old doctor of traditional Chine se medicine professor Guo Ziguang after 60 years of clinical validation summed up experience in the therapy of CRF dohave remarkable curative eff ect.Therefore,to research fushengong decoction renal protective effect of CRF rats,can find new therapeutic targets for Chinese medicine treatment of CRF provide experiment albasis,so,This topic research to digdeeper into national medical experts clinical experience is of great importance to masterthe clinical practical value, social economic value and scientific research value.Objective: To investigate fushengong decoction renal protective effect of CRF rats, this topic detection of CRF rats renal function,and expression of α-SMA,E-cadherin and Nephrin in kidney tissues.To provide an experimental basis for the development of a safe and effective treatment of CRF,Respectively from the renal fibrosis, protect the renal tubules and foot cells, exploring it protection mechanism,in order to develop a safe and effective proprietary Chinese medicine to provide experimental basis for the treatment of CRF.Methods: 55 male SD rats were randomly divided into five groups: control group, model group, and low, medium and high Fushengong Decoction dose groups(11 in each group). Rats in control group were fed with standard chow, while the other four groups were fed with 0.5% adenine feed stuff to make the CRF model. After the models were successfully made, all rats received standard chow. Then, rats in control and model groups received intra-gastric normal saline(NS) by 20ml/kg.d for 30 d, while those in low, medium and high Fushengong Decoction dose groups received Fushengong Decoction at the dose of 4 g/kg, 8 g /kg and 16 g/kg respectively, once a day for 30 d. After the last administration for 12 h fasting, the rats were anesthetized by 20% urethane 5 mL?kg-1, the eye blood serum was separated and preserved the supernatant, and finally the rats sacrificed by cervical dislocation. Histomorphology of glomerulus and renal interstitial fibrosis were detected by HE staining and and Masson staining. The expression of α-SMA and E-cadherin were detected by immunohistochemistry and western blotting.the expression of Nephrin was detected by Immunofluorescence and RT-PCR.Results:The expression of α-SMA in nephridial tissue were increased significantly in the model group compared to those of the control group(P<0.05), After the treatment of Fushengong Decoction, compared with the model group, the expression of α-SMA were decreased and E-cadherin and Nephrin were increased significantly(P<0.05). Light microscopy showed no significant pathological changes in renal tissue of normal rats while glomerular atrophy deformation, capillary congestion significantly, renal tubular epithelial cell degeneration, loss, renal tubular epithelial incomplete, showing protein casts and red blood cells, diffuse inflammatory cell infiltration and fibrosis in the renal interstitium in the model group.Figure1 show, while pathological changes were significantly alleviated in low, medium and high Fushengong Decoction dose groups.Conclusion: Fushengong decoction protection of CRF rats can significantly improve the renal fibrosis and protective renal tubular and podocyte in rats with CRF by reducing the expression of α-SMA and promotinging the expression of Nephrin and E-cadherin in nephridial tissue. |
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