Screening Of Active Ingredients With Promotion Of Intestinal Propulsion And Their Preliminary Pharmacodynamic Reasearch From Portulace Oleracea L. And Folium Mori

Constipation is a key issue that affects people’s lives and work. With the development of social economy and the accelerated pace of life, the incidence of constipation are increasing year by year. Pathophysiological mechanism of constipation is not fully understood, and it may be lack of colonic motility and intestinal disorders. Currently, peristaltic inhibition model and defecation dysfunction model are widely used to complete pharmacodynamic evaluation of drugs for treatment of constipation. It has been reported that some traditional Chinese medicines have action on lubricating bowel and promoting defecation. Nine Chinese herbal medicines were selected. By using peristaltic inhibition mice model, active ingredients with the promotion of intestinal propulsion were screened. Dose-effect relationship study and defecating function evaluation were also conducted.Firstly, loperamide-induced peristaltic inhibition mice model and bowel dysfunction mice model were successfully prepared. Then, Cassia obtusifolia L., Juglans regia L., Cannabis sativa L., Prunus persica(L.) Batsch, Prunus mume(Sieb.) Sieb.et Zucc., Portulaca oleracea L., Folium Mori, Cichorium intybus L. and Perilla frutescens(L.) Britt. were selected, and their aqueous extracts were obtained. Using peristaltic inhibition mice model, evaluaton of enterokinesia enhancement effect was completed, and result showed that aqueous extracts of Portulaca oleracea L. and Folium Mori can obviously promote small intestinal propulsion. Portulaca oleracea L. and Folium Mori were then extracted successively with petroleum ether, ethyl acetate and n-butyl alcohol to prepare four active fractions. The result of evaluaton suggested that water fractions of Portulaca oleracea L. and Folium Mori have a stronger role in promoting intestinal propulsion than other active fractions. Finally, through water extraction and alcohol precipitation, Sevage method, acid extraction and alkali precipitation, and resin purification, polysaccharides, removal of protein polysaccharides, alkaloid, flavonoid, water elution and 70% ethanol elution washing off resin of Portulaca oleracea L. and Folium Mori were respectively prepared. The study of their enterokinesia enhancement effect suggested that removal of protein polysaccharides from Portulaca oleracea L. was superior to other active ingredients and showed a certain dose-effect relationship. Furthemore, effect of removal of protein polysaccharides from Portulaca oleracea L. on defecation was also investigated. And it had a positive effect on purgation and played a similar role with positive control drug.This research demonstrates for the first time that removal of protein polysaccharides from Portulaca oleracea L. can obviously promote intestinal propulsion and defecating function, and shows a dose-effect relationship, offerring reference for further development of constipation treatment products.