Studies On The Chemical Constituents Of Portulaca Oleracea L

The dry eniire grass of Portulaca oleracea L. soaked with ethanol, after filtration andconcentrate. Preparation extract dispersed with water, then it was re-extracted with n-Hexane,Ethyl acetate and n-Butanol, respectively, to obtain the corresponding extracts, which of thepercentage accounted for the grass of Portulaca oleracea L. extractive was0.99%、0.45%and0.35%. After separating the three extracts above by silica gel column chromatographyand HPLC. By using the modern preparative chromatography techniques, twenty-onecompounds were isolated from the n-Hexane, EtOAc extracts and n-BuOH extracts. On thebasis of classical chemical methods and modern spectrometric approaches, the structures ofthem were established. The compounds include25-hydroxy-dammarane-3-ketone (1),β-sitosterol (2), linoleis monoglyceride (3), Three linoleic acid glycerol ester (4), lupeol (5),3-acetyl-Ursane-12-ene (6),12,20-two hydroxyl-dammarane-25(27)-ene-3-ketone (7),3-acetyl-oleanane-12-ene (8), DL-epiloliolide (9), n-cluytyl alcohol (10),1,3-dual-glyceridesof palmitic acid (11), n-hexacosyl alcohol (12),13-hydroxy-(13R)-phaeophytina (13),13-hydroxy-(13S)-phaeophytina (14), α-palmitic acid monoglyceride (15),N-transferuloyltyramine (16), scopoletin (17), uracil (18), β-sitosterol-glucopyranoside (19),allantoin (20), sucrose (21). The compounds of1、6、7、8、9、18were isolated from this plantfor the first time..Activity tests show that, ethyl acetate and petroleum ether extractive of grass ofPortulaca oleracea L to Tomato frost cactorum、pachrymans、Fusarium oxysporium、Rhizoctonia solani、 Magnaporthe grisea and Fusarium oxyspirum fsp.niveum withoutantibacterial activity, but the n-Butanol extractive has a strong antibacterial activity to Tomatofrost cactorum, and dependencies concentration.