Construction And Evaluation Of Three Dimensional Permeable Hollow Fiber Cartilage Tissue Engineering Woven Scaffold

Degenerative arthritis is a common disease which is one of the main causes of joint pain. The occurrence of the disease often with injury, excessive weight, infection or inflammation caused by destruction of articular cartilage and subchondral bone necrosis. It has a direct impact on the life quality of the patients because of the main performances of joint swelling and pain and patients with impaired mobility. The disease is related to age and it happens rarely before 40. With the growth of age, the incidence increases gradually, most people have degenerative arthritis after 70.With the China aging intensifies, the incidence of degenerative arthritis becomes more and more common and people are paying more attention on the treatment.Degenerative arthritis is mainly caused by disease or injury of cartilage, so the goal of treatment is to repair cartilage tissue. Due to the lack of the vascular system, lymphatic system and nerve injury, self repair ability the articular cartilage is poor. The damage cannot be repaired by itself when the damage diameter is greater than 4mm. Tissue engineering provides a technical means of tissue regeneration, which will change the traditional surgical treatment mode of trauma to trauma repair, and enter a new stage of wound healing with no damage. In order to repair damaged cartilage tissue, cartilage tissue engineering also comes.In this study, the experiments were based on the premise that cells could be cultured in bioreactor. The mechanical properties of polypropylene hollow fiber, polysulfone hollow fiber and PLA filament were compared respectively, then polypropylene hollow fiber was selected as weft yarn and PLA filament was used as warp yarns and Z yarns to weave three-dimensional scaffolds for tissue engineering. The water contact angle of polypropylene hollow fiber and PLA filament and the physical and chemical changes of the surface before and after cell culture were tested. Itshowed that the polypropylene hollow fiber and PLA filament were suitable for cartilage cell culture. In order to achieve the growth of cartilage cells in the scaffold, the circulation system was set up, and the toxicity of the sealant was tested, which indicated that the circulation system was suitable for the growth of cartilage cells. By SEM observation and measurement of polypropylene hollow fiber surface micropores and after circulation of glucose solution, urea, lactic acid solution and serum(FBS) in the scaffold, the results showed that different size molecules can go through the micropores and cells could not. It was that polypropylene hollow fiber had selective permeability and different size molecular go through the micropores at the same time, which has nothing with the size of the molecules..The cartilage tissue engineering scaffold was constructed by the method of three-dimensional weaving, and the inner circulation of the cartilage tissue was realized by selecting permeable hollow fiber as the blood channel. In order to mimic and compare the growth of cartilage cells in vivo, the proliferation and distribution of cartilage cells on the scaffolds were observed with circulation system and no circulation system. It was found that the cartilage cells proliferation in circulation system was significantly better than that of no circulation system by MTS test. Also it was found that most of the cells attached on the PLA filament and polypropylene hollow fiber with a small amount of cell attachment by laser confocal scanning microscope. Therefore, the hollow fiber in the circulation system effectively acts as a blood channel to achieve the inner circulation, which significantly promotes the proliferation of cartilage cells in the scaffold.