Preparation And Properties Of Sodium Composite Fiber Material KGM / Hyaluronic Acid

How to repair articular cartilage injury has been a problem. The development of tissue engineering technology provides a new choice for the cartilage regeneration and repair. The selection and preparation of cartilage scaffolds is the key to cartilage tissue engineering. Therefore, an ideal cartilage scaffold is particularly important. Konjac glucomannan is a straight-chain plant polysaccharides with branched-chains, which has such advantages as good cross-linking, simple molding, certain strength and toughness, etc. Hyaluronic acid as an important cartilage matrix proteoglycan plays an important role in maintaining the chondrocyte phenotype and promoting cartilage mechanism secretion, etc. Therefore, intention to make use of these advantages of the two materials were the main gaol of this thesis. Compobinasion of the two together effectively, through the wet spinning method and programmable two-dimensional mobile platform to prepare the porous fiber membrane materials, which were expected to become one of the potential cartilage tissue engineering scaffolds.KGM and SH were used as raw material, through the wet spinning method and programmable two-dimensional mobile platform to prepare the KGM/SH fiber membrane materials. First, the lactic acid solution and microwave pretreatment were used to modify the composite colloidal to obtain the spinning solution with a suitable viscosity; and then, a porous fiber membrane material was prepared through the wet spinning method and programmable two-dimensional mobile platform to array the fibers regularly, then through a complex cross-linking process, and subsequent processing to obtain the final the KGM/SH fiber membrane materials. The KGM/SH fiber membrane preparation process was researched through the orthogonal pilot program L9(34). The optimal formula for the preparation of the fiber membrane was as follows:4.00g konjac glucomannan,1.25g sodium Hyaluronate,1.5ml lactic acid,5.0min microwave treatment and400ml distilled water. The fiber membranes were obtained with the optimized condition. The viscosity of spinning solution was3460mPa· s. The water absorption of membrane was946%. The thickness of the membrane was74.3μm. The tensile strength was15.9MPa and elongation at break was4.7%. The degradation rate in12weeks was60.4%-79.2%.XRD、TGA-DTA、FT-IR were applied to characterize the KGM/SH fiber membranes. The crystallinity, thermal stability, chemical bonding of the fiber membranes and the interaction between KGM and SH were investigated. It was revealed that the degree of crystallinity Of KGM/SH fiber membranes after cross-linking treatment increased significantly, with24.66%crystallinity. TGA-DTA results showed that the cross-linking treatment can improve the thermal stability of the KGM/SH fiber membranes. FT-IR results showed that the hydroxyl group of KGM and the carboxyl group of SH occurred esterification reaction, there are a large number of hydrogen bonding between KGM and SH, the acetyls peak in KGM were disappear after cross-linking treatment.The cytocompatibility was investigated by co-cultured of human umbilical cord mesenchymal stem cells and KGM/SH fiber membranes. The biological activity of the fiber membranes was observed by SBF mineralization experiments. The cytotoxicity was also tested by MTT assay. It was revealed that the fiber membranes had good cytocompatibility, biological activity and cytotoxicity, which indicated that the membrane has a good prospect as an potential cartilage scaffold after seriously improved.