Establishment Of Immune Chromatography For The Detection Of Autoantibodies And A Plasmonic ELISA For Heavy Chromium Ions Detection

Objective: Establish an effective, fast(5 ~ 8 min), simple and high sensitivity colloidal gold immunechromatography test paper of the technology, it can be applied to clinical detection of autoantibodies in serum. Ensure timely and effective feedback of testing results and auxiliary diagnosis for autoimmune diseases. Seize the golden time for clinical treatment.Methods: Establishment of immune chromatography to detect Anti SSA/SSB antibodies, staphylococcus aureus protein A(SPA) was labled with Gold nanoparticles(Gold nanoparticles, Au NPs) to prepared Au NPs-SPA and then dispensed on the conjugate pad. Antigen(SSA/SSB) was dispensed on a specific lo Catalaseion of nitrocellulose membrane(NC) membrane as Test line(T line), goat polyclonal antibody was dispensed on Another specific lo Catalaseion as Calibration line(C line). To sample pad, combination pad, NC membrane, serum sample dilution buffer etc comprehensive optimization and complete the colloidal gold immunechromatography strip(ICS)assembly. The establish(ICS)was used to detect serum samples(the second people’s hospital of Guangdong province) and the test result were compared with the current hospital clinical commonly used detection methodsResults: The total coincidence rate between the ICS for anti SSA antibody and the clinical commonly used method(LIA)was 95.12%; The total coincidence rate of the ICS for anti SSB antibody and the clinical commonly used method(LIA)was 97.82%.Conclusions: This study to establish ICS for antibody of SSA, SSB antibody detection of clinical serum samples have high coincidence rate with the LIA and ELISA test results. The ICS have advantages of simple, rapid(5 ~ 8 min) and sensitivity, and can be used as auxiliary diagnosis methods to AID.Objective: Estable a new strategy and can the naked-eye observation test results plasmonic sensor, The test signal intensity was positively related with analytes concentration, this method results interpretation is simple, no instrument support and has good potential for appli Catalaseions in resource-constrained areas.Methods: Using hydrogen peroxide(H2O2) etch triangular silver, makes the triangular silver size and shape change and show the special optical characteristics(change from blue to purple). According to this phenomenon, we established a plasmonic ELISA to detect Cr(III) in real water samples. On the basis of competitive ELISA, through Catalase labled Ab2, using H2O2 and Triangular silver nanoprism(triangular Ag NPRs) as chromogenic substrates(Catalase regulate the concentration of the residual H2O2 and regulating the level of triangular Ag NPRs etched. The LSPR change intensity(color and absorption spectrum) of triangular Ag NPRs change intensity were positively correlated with Cr(III) concentration. The feasibility, the sensitivity and specificity of this method to detect the actual water samples were evaluated, the test result were compared with the ICP-MS.Results: The Plasmonic biosensors for Cr(III) detection was 6.25 ng/m L as judged by the naked eye, the specificity and repeatability were good. The sensitivity for Cr(III) was 3.13 ng/m L with a linear range from 3.13 ng/m L to 50 ng/m L based on the LSPR blueshift of the Ag NPRs.Conclusions: The Plasmonic biosensors can be used for limited resources without expensive instruments and can also do a preliminary test of heavy metal concentration in the actual samples.