Study On Secondary Metabolites Of Two Marine Actinomycetes

For the purpose of finding bioactive compounds with unique structures, two marine actinomycetes including Dietzia maris and Micrococcus yunnanensis, were chosen for the further research by bioactive and chemical screening. These two actinomycetes were subjected to systematic investigation for secondary metabolites by modern chromatographic methods. Structures of isolated compounds were elucidated by analyzing their 1D and 2D NMR and MS data and comparison of chemical and physical data with authentic samples reported in literatures.As a result, seventeen compounds were isolated and indentified as cyclo(Val-Pro)(1), 2-(6-hydroxy-3-oxahexyl)-1,3-dioxane(2),(E)-3-(methylthio) acrylic acid(3), 4-hydroxyphenylacetic acid(4), cyclo-(Pro-Met)(5), cyclo-(Pro-Ile)(6), cyclo-(Pro-Leu)(7), cyclo(Pro-Phe)(8), 2-(5-amino-2-hydroxyphenyl) acetic acid(9), indol-2-hydroxy-1-ethanone(10), pyrazin-2-ylmethanol(11), pyrazine-2,6-diyldimethanol(12), cyclo(Pro-Tyr)(13), indole-3-methylethanoate(14), indole-3-carboxadehyde(15), indoly-3-carboxylic acid(16), indole-3-acetic acid(17). Compounds 1~10 were isolated from Dietzia maris and compounds 11~17 were yielded by Micrococcus yunnanensis.In conclusion, seventeen compounds were isolated from two marine actinomycetes. Compounds 1~10 were isolated from Dietzia maris for the first time. Compounds 11~17 were isolated from Micrococcus yunnanensis for the first time.All seventeen compounds isolated from this reacherch were tested for their inhibitory activity on human lung cancer cell H1299 and the results of isolated compounds were negative.