Effect Of Sinomenine On Apoptosis In Renal Tubular Epithelial Cells Of Rats Subjected To Renal Ischemia-reperfusion

Objective:To evaluate the effects of sinomenine on epithelial cell apoptosis in kidney tubules of rats models of ischemia reperfusion injury. Discussing what roles sinomenine play and probable mechanism in the kidney ischemia- reperfusion injury., and hoping that could provide the experiment evidence and theoretical basis on the clinical application of sinomenine prevention kidney ischemia-reperfusion injury.Methods : Fifty four male Wistar rats,weighing 180-220 g,were randomly divided into 3groups,each group include 18 male Wistar rats,and the following are specific groups : group of sham operation(group S), group of ischemia-reperfusion injury(group IRI), group of sinomenine(group SIN). IRI group and SIN group of renal ischemia-reperfusion injury models were made by Renal ischemia inducing occlusion of the left renal pedicle for 45 min and restoring perfusion.Sinomenine was infused at 60mg/kg via the abdominal cavity starting from 30 min before reperfusion in group SIN,while the equal volume of normal saline was given instead of sinomenine at the same time in groups S and IRI. Six male Wistar rats in each group were randomly chosen, after reperfusion, the right kidneys immediately were removed. we got 2ml of blood to measure serum creatinine(Cr) and urea nitrogen(BUN) at 0.5h,6h,24 h after reperfusion(T1-3), before blood collection the left renal specimens were obtained to observe kidney pathology results by light microscopic, detect the expression of p-JNK and Caspase-3 protein by immune-histochemistry and test the apoptotic rate of renal tubular epithelial cells by TUNEL.Results: Compared with S group, the level of Cr and BUN in IRI group and SIN group were increased(P < 0.05), the expression of p-JNK were significantly up-regulated(P < 0.05), the expression of caspase-3 and the apoptotic rates of epithelial cell were no significant in T1(P>0.05),the expression of caspase-3 and the apoptotic rates of epithelial cell were up-regulated in T2 and T3(P < 0.05);Compared with IRI group, he level of Cr and BUN in IRI group and SIN group were lower(P < 0.05), the expression of p-JNK in IRI group and SIN group were down-regulated(P < 0.05), the apoptotic rates of epithelial cell were lower(P < 0.05). Observed under light microscope, the renal pathological injury in SIN group was obviously lighter than IRI group.Conclusion:Sinomenine could attenuate ischemia reperfusion injury of rat kidney models, and improve the renal function at some level; the mechanism of Sinomenine relieving renal ischemia-reperfusion injury may be related to inhibiting the expression of p-JNK signal transduction pathway and reducing apoptosis rates of the renal tubular epithelial cells.