Effects Of Probiotics On Intestinal Immune And ICC Of Colon Cancer Patients

Objectives: To investigate the effect of probiotics on the intestinal immune and ICC of colon cancer patients, to provide theoretical evidence for clinical application of probiotics.Methods: â‘ The colon cancer scheduled to undergo right hemicolectomy of hospitalized patients as research subjects, randomly divided into control group and the probiotic group, probiotic group giving probiotics drug oral 3 days before surgery, and control group was not special treatment. â‘¡Comparison of the two groups research objects postoperative infection, postoperative 24 h WBC. â‘¢ HE staining to observe research object ileum and colon inflammatory cell infiltration and goblet cell changes. â‘£Immunohistochemical detection expression of IL-10, IL-17, IL-22 of research objects’ ileum and colon tissue, and calculate mean density. ⑤Enzyme-linked colorimetric test the concentration of D-lactic acid of research objects’ blood. â‘¥Comparison of the two groups postoperative gastrointestinal recovery, and immunohistochemistry to detect the expression of c-kit of research objects’ ileum and colon tissues.Results: â‘ 3 cases of control group and 2 cases of probiotic group had postoperative infection. 24 h after operation the control group and the probiotic group WBC were(10.26±2.81)×109/L and(9.40±2.12)×109/L. Percentage of neutrophils were(81.87±8.45)% and(77.16±7.40)%. No significant difference between them(P>0.05). â‘¡Compared with the control group, probiotic group infiltration of inflammatory cells in Ileum and colon tissue was mild. The number of goblet cells was(5.93±1.44)×10-5 and(6.70±1.07)×10-5 more than control group(4.85±1.37)×10-5(P<0.05)and(5.76±1.26)×10-5(P<0.05)respectively. â‘¢ IL-10 mean density of probiotic group in ileum and colon tissue were 0.1550±0.0308 and 0.1713±0.0396, significantly higher than control group 0.1307±0.0337(P<0.05) and 0.1442±0.0303(P<0.05). IL-17 mean density of probiotic group in ileum and colon tissue were 0.1461±0.0339 and 0.1323±0.0283, significantly lower than control group 0.1751±0.0378(P<0.05) and 0.1552±0.0302(P<0.05). IL-22 mean density of probiotic group in ileum and colon tissue were 0.1184±0.0201 and 0.1212±0.0225,significantly lower than control group 0.1374±0.0272(P<0.05)å'Œ 0.1480±0.0385(P<0.05). â‘£D-lactate concentration of probiotic group is(603.6188±56.8916)μg/L, significantly lower than control group(885.7639±106.0899)μg/L(P<0.05). ⑤There is no difference between control group(4.45±1.05)day and probiotic group(4.55±1.39)day in defecation after surgery(P>0.05). Expression of c-kit in ileum and colon tissue of probiotic group was significantly higher than that in control group.Conclusions: â‘ Probiotics reduce the extent of inflammatory cell infiltration in the ileum and colon intestinal mucosal tissue of colon cancer patients, protect the number and normal function of goblet cells. â‘¡Probiotics can increase the expression of inhibition inflammatory cytokine IL-10 in ileum and colon tissue of colon cancer patients, reduced the expression of inflammatory cytokines IL-17, IL-22. Thereby reducing the local intestinal inflammatory response. â‘¢ Probiotics can reduce blood D-lactate levels in patients with colon cancer. Probiotics can reduce the permeability of the intestinal mucosa of colon cancer patients, enhanced mucosal barrier. â‘£Probiotics can increase the expression of c-kit in ileum and colon tissues, increase the number of ICC, it may be a factor in probiotics can regulate gastrointestinal motility.