| Objectives:Using ELISA and CBA methods to detect different serum AQP4-Ab in patients with NMO-ON to accuracy evaluation of ELISA.Detection of serum cytokines (IL-1β、IL-6、IL-6R、IL-10、TL-17) content using high throughput protein chip to preliminary discussion NMO-ON patients recovery between the cytokines and possible relationship, for clinical treatment and predict prognosis, prevention and of optic nerve myelitis associated optic neuritis mechanism to provide some experimental basis.Methods:36 cases of patients diagnosed as optic neuropathy were collected from the Department of Ophthalmology,General Hospital of PLA.The titer of AQP4-Ab were measured by CBA method and ELISA method.To compare the positive rates of the two methods and observe whether the titer of serum AQP4-Ab was correlated with the severity of the disease.The patients were divided into two groups,10 patients with good vision recovery group (group GR),6 cases of poor vision recovery group (group UR) and 2 health people(group HC).Detection of IL-1β、IL-6、IL-6R、IL-10、IL-17, compared with each of the different groups of the detection value of each cell cytokine. Pearson correlation analysis was performed on the cytokines in each group, and the significance of these cytokines in the progression and prognosis of the disease were discussed.Results:AQP4-Ab were detected by ELISA. Results showed that NMO-ON group (GR±UR group) (62.735±27.242) and simple optic neuritis group(HC group)(1.514 ±0.863) between group comparison, P<0.01, GR group (63.920±31.173) with UR group (60.760±21.675) comparison, P> 0.05, the difference without statistical significance. Compared with CBA method, P=1, the difference was not statistically significant.IL-1β:GR group (58.682±38.921),UR group (74.019±34.816) and HC group(52.600±0.619).the difference was not statistically significant.IL-6:GR group(214.394±86.329),UR group(206.944±103.976) and HC group(118.734±43.237). The mean difference among the three groups were not statistically significant.IL-6R:UR group (2310.766±173.858) and GR group (1881.391±206.023) (P= 0.004) and HC group (1881.391±206.023)(p=0.046),the differences were statistically significant, the GR group and HC group differences without statistical significance (p=0.667).IL-10:GR group (10.844±4.949)、UR group (10.786±7.225) and HC group (7.352±4.090), prevalence group and healthy group’s mean are differences, pairwise comparison, the difference was not statistically significant. There was no significant difference between any two groups.IL-17:The fluctuations in the GR group (9.7815±7.579), UR group (9.448+ 6.779) and the prevalence group (9.656±7.057) is larger.There is no statistical difference compared with each group.Conclusion:1.The CBA method is the gold standard for the detection of AQP4-Ab at present,but the ELISA detection is also a kind of feasible method.2. IL-6R plays an important role in judgment of prognosis,It maybe another way of causing disease.We can try to treat the neuromyelitis optic associated optic neuritis as the therapy target. |
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