| Objective:Artemisinin is one and a half times terpene lactone compounds, it is characterized by having a peroxide bridge structure.After this,dihydroarteannuin (Hao Jiami), artesunate and other artemisinin derivatives were developed gradually.In this paper, we will study the inhibitory effect of artemisinin and its derivatives on lung squamous cell carcinoma cell line YTMLC and SK-MES-1 tumor cells by in vitro tumor cell experiment,and explore the mechanism of its inhibition.Research methods:Technique of tumor cells culture in vitro was used,by FCM analysis, tumor cell screening in vitro and confocal microscopy,study the inhibitory effect of artemisinin and its derivatives on lung squamous cell carcinoma cell line YTMLC and SK-MES-1 tumor cells.Results:Artemisinin and its derivatives have obvious inhibition effect on SK-MES-1 cells.Through experiments and comparison, it is found that the number of colony formation is significantly reduced, and the difference is statistically significant (P<0.05).At the same time, it is not only a significant reduction in colony formation, but also in the cell level, the colony is relatively loose, each cell state is relatively poor.The inhibition rate was significantly increased with the increase of the concentration of the drug.The control group cell specific fluorescence staining was observed,Only has a vague background fluorescence.P-JNK level was higher than the control group, the difference was statistically significant (P< 0.01).Test different concentrations of artemisinin and its derivatives effecting on YTMLC cells Lasting for 24、48、 72 hours separately.The inhibitory effect of artemisinin on the growth of YTMLC cells was closely related to the concentration and time.The study found that the greater the drug concentration, the longer the time of action, the inhibition rate is also significantly improved.Conclusion:Artemisinin and its derivatives dihydroartemisinin on lung cancer cell lines YTMLC and SK-MES-1 cancer cells significantly inhibited. |
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