| Objective:Experimental study that explores the function of Liver-Clearing Kidney-Nourishing Chinese medicine (Zhenjian granules II) on improving metabolic disturbance of spontaneously hypertensive rats that have been fed up with high lipid diet, through its regulation on the secretion of various adipokines, including serum leptin, resistin, adiponectin and visfatin. This study aims to lay foundation for the unique multi-target effect and multi-link adjustment advantage of Chinese medicine as a strategy in clinically practice for cardiovascular risk management caused by high blood pressure.Method:23 4-week-old male spontaneously hypertensive rats (SHRs) were chosen. All rats were randomly allocated into different groups, with 5 rats in control group, and 6 rats each in model group, zhenjian low-dosage group, and zhenjian high-dosage group. The control group were fed with normal meal, whereas the model group, the zhenjian low-dosage group and the zhenjian high-dosage group were all fed with high lipid meal. After one week of high lipid meal feeding, the zhenjian low-dosage group were also given low dosages of Zhenjian Granule Ⅱ (11.25g/kg/d) suspension through intragastric administration. Similarly, after one week of high lipid meal feeding, the zhenjian high-dosage group were given high dosages of Zhenjian Granules II (22.5g/kg/d) through intragastric administration. The control group and the model group were given equal amount of cellulose sodium as placebo through intragastric administration. A total of 9 weeks of intragastric administration were performed. Blood serum samples were taken 2 times from the rats, first after 4 weeks and then after 9 weeks, each time with 12 hours of fasting to test various metabolic indexes including triglyceride (TG), cholesterol (CHO), high density lipoprotein-cholesterol (HDL-C), fasting glucose and fasting insulin, and expression of various adipokines including adiponectin ELISA, visfatin, leptin, and resistin. By utilizing the software SPSS 13.0, the results of the above experiment were statistically analyzed.Result:1. SHRs that were fed with high lipid diet showed distinct disturbance of glucose metabolism. After 9 weeks of intervention by liver-clearing kidney-nourishing Chinese medicine, the fasting insulin level of the zhenjian low and high dosage groups markedly decreased comparing to the model group. The fasting glucose/fasting insulin ratio of the zhenjian low and high dosage groups markedly increased comparing to the model group. The differences are all statistically significant (p<0.05, p<0.01).2. The feeding of high lipid diet to SHRs lead to distinct disturbance of lipid metabolism. After 9 weeks of intervention by liver-clearing kidney-nourishing Chinese herbs, the TC value of zhenjian high-dosage groups and the TG value of the zhenjian low-dosage group showed marked reduction. The HDL-C value of the zhenjian high-dosage group comparing to before treatment showed marked increase. The differences all reached statistical significance (p<0.05).3. Regarding serum adiponectin level before treatment, the control group is distinctively higher than the model group and the zhenjian low-dosage group; at the same time, the high and low dosage groups are distinctively higher than the model group(P<0.01). Regarding adiponectin level after treatment, zhenjian low and high dosage groups and the model group are significantly lower than the control groups (P<0.01), yet the zhenjian low and high dosage groups are significantly higher than the model group(P<0.01).4. Regarding resistin level before treatment, the model group, zhenjian low-dosage group and zhenjian high-dosage group are all significantly higher than the control group (P<0.01). Regarding resistin level after the treatment, the model group and zhenjian low and high dosage groups are all significantly higher than the control group(P<0.01), yet the zhenjian low and high dosage groups are significantly lower than the model group. ((P<0.01).5. Before treatement, serum leptin level of the model group is distinctively higher than that of the control group (P<0.05). Zhenjian low and high dosage groups although higher than control group, but their differences show no statistical significance. After treatment, serum leptin level of the model group, zhenjian high-dosage group and zhenjian low-dosage group are significantly higher than the control group (P<0.05). The zhenjian high and low dosage groups are significantly lower than the model group (P<0.01).6. Regarding serum visfatin level before treatment, the model group is markedly higher than the model group and the zhenjian low dosage group. After treatment, visfatin level is the highest among all, yet the differences between the control group and the zhenjian low and high dosage groups has no statistical significance.Conclusion:SHRs fed with high lipid diet may develop metabolic disturbances, at the same time, significant secretion disturbances of various adipokines may occur as well. Clinical manifestation include distinctive increase in visfatin, resistin and leptin levels and distinctive decrease in adiponectin level. Intervention of liver-clearing kidney-nourishing Chinese medicine (Zhenjian Granule II) shows regulatory effect for the secretion of adipokines for SHRs that were fed with high lipid diet. |
PDF Full Text Request