Study Of The Effects Of P53 Induced Uc.106+ On B Cell Lymphomagenesis Cell Line 38B9

[Aims]Patients of Burkitt’s lymphoma, the prognosis of which is poor, are mainly children and adolescents. At present, many studies have found that long noncoding RNAs(lncRNAs) is closely associated with the development and progression of human tumor diseases.Thus, lncRNAs are gradually applied to the prevention and treatment of human cancers.Ultraconserved Regions(UCRs) are a kind of lncRNAs. Our research group had used mouse bone marrow cells with controllable expression of P53(P53-ER KI) and cells with c-myc overexpression to establish a mouse model of B-lymphoma. Injection with 4-hydroxytamoxifen to activate P53 can induce most of the tumor cells apoptosis. The expression of ultra-conserved RNAs were detected in tumor cells with P53 activation using microarray.Some ultra-conserved RNAs’level in P53 activated tumor cells dropped markedly.This study aims at verificating the expression of 5 of these UCRs and selecting one of them uc.106+ to explore the effect of it on the growth of B cell lymphoma and the potential mechanism.[Methods]1.The expression of 5 UCRs in tumor cells with P53 activation were detected using real-time quantitative PCR. Among the 5 UCRs, we selected uc.106+ for further study.We used real-time quantitative PCR to detect the expression of uc.106+ in B lymphocyte cells and B lymphomas cells(38B9) of mice.2.To investigate the influence of uc.106+ on the B-lymphoma, we constructed recombinant plasmid of uc.106+-pMSCV-PIG and gained the the retrovirus by packaging cells.Then we transfected B cell lymphoma with the retrovirus to overexpress uc.106+.Cell counting was used to detect the cell proliferation.3.By injecting 38B9 cells infected with virus produced from empty vector or from uc.106+-pMSCV-PIG,we made a tumor-bearing mice.We took out the tumor after 2 weeks and measured the size and weight of the tumors. Next, we extracted the total protein of the tumor to detect the expression of OLAl by Western Blot.[Results]1.Rt-qPCR test results showed the expressions of uc.3+、uc.106+、uc.172+、uc.263+ and uc.281+A in B-cell lymphoma with P53 activation are significantly reduced.We selected uc.106+ for further study about its impact on B-cell lymphoma.Compared with B lymphocyte cells the expression of uc.106+ is lower in B cell lymphoma cells.2. Cell counting experiments showed that the cell growth of 38B9-MSCV-uc.106+group slow down compared with 38B9 and 38B9-MSCV group.3. Compared with 38B9 and 38B9-MSCV group,the weight and the size of the tumors with overexpression of uc.106+ are decreased after two weeks. In addition,the expression of OLA1 is reduced in 38B9-MSCV-uc.106+tumors.[Conclusions]1.These results support that uc.106+ might restrain the growth of B cell lymphoma growth.2.uc.106+ inhibitting B cell lymphoma might related with the downregulation of OLA 1.