Analysis On Drug Resistant And Homology Of Blood Stream Infection Caused By Serratia Marcescens From An Intensive Care Unit

Objective:This investigation was aimed to provide a basis for the controlling and treatment to blood stream infection caused by Serratia marcescens through analyzing the homology and drug resistant genes of the isolates from intensive care unit (ICU) of Shaoxing County Central Hospital.Methods:From June first to September thirtieth,2013, we collected the cultured Serratia marcescens strains from the blood stream infected patients in the ICU, and cultured the isolates from the hands of health care workers working in the ICU. Seventeen strains were collected, and the drug susceptibility test was performed. The PCR technology was used to amplify the common drug resistant genes, and the pulse-field gel electrophoresis (PFGE) was carried out for the homology analyzing to all the isolates. All the clinical data of the patients were collected, the method of Spearman correlation was used for the statistical analysis.Results:The 17 strains were resistant to the first and second generation cephalosporin, gentamicin and ciprofloxacin, sensitive to amikacin and ceftazidime. The drug resistant rate to carbapenems was from 11.76% to 35.29%. It showed that the TEM gene was amplified from one of the isolates. The PFGE result showed that all the isolates had the same phenotype.Conclusion:Serratia marcescens is an important pathogen. It was able to spread in the hospital, and it was resistant to multiple antibiotics. Clinical physicians should choose the proper antibiotics for the patients according to the result of the drug susceptibility test, and strengthen the infection control to avoid the cross spreading and the outbreak of resistant strains.