| As one of Chinese traditional folk tonic, red jujube contains a lot of protein, polysaccharide and fat as well as other important biological active substances including flavonoids, alkaloids and saponins, etc. It is supported by traditional Chinese medicine that often eating red jujube in proper quantity can improve symptoms of physically weak, disharmony between spleen and stomach and anemia, etc. Modern medical study found that red jujube also shows good biological activities in aspect of against oxidation, anticancer and inhibiting tumor. Deep processing of red jujubes will produce a large number of wastes jujube peels which contains rich red pigment of jujube peel. Studies have confirmed that jujube peel red pigment contains rich antioxidants, such as gallic acid, catechin, rutin and other functional ingredients. These substances contain several biological activities of removing the free radicals in body, antivirus, antitumor and anti-inflammation, which has positive effect on people’s health and play an important on research value. This experiment establishes experimental model on animal with injury liver of hypoxia tolerance at normal pressure and restraint stress to test the change of antioxidant enzymes and antioxidants in the body of experimental animals when stress happens after the gavage of jujube peel of red pigment by adopting the seven-week male ICR mice and discusses the protection jujube peel red pigment on injury liver under hypoxia tolerance at normal pressure and restraint stress.(1) Establish animal experimental model of hypoxia tolerance at normal pressure to observe the change of surviving time and oxygen consumption of the mice after the gavage of jujube peel red pigment under the condition of normal pressure hypoxia. Adopt respectively the method of NBT to test the activity of SOD in plasma and thiobarbituric acid method and GRIESS chemical method to test the content of MDA and NO in brain tissue and use spectropho to metric method to test the activity of GSH-PX and LDH in brain tissue. The experimental results show that surviving time and oxygen consumption of the mice after the gavage of jujube peel red pigment under the condition of normal pressure hypoxia significantly rise and decrease (P< 0.05). Jujube peel red pigment can effectively reduce the content of MDA, NO and activity of LDH (P< 0.01) in brain tissue, significantly improve the activity of SOD in plasma and activity of GSH-PX in the tissue (P<0.01). Jujube peel red pigment has obvious improvement on oxidative stress injury of mice under condition of normal pressure hypoxia by increasing the antioxidant capacity of the body and reducing the degree of lipid peroxidation.(2) Establish animal experimental model of constraint stress with constraint model group and blank control group. Make corresponding constraint processing on constraint model group and no any processing on blank control group. After the constraint processing, test respectively the MDA content and T-AOC level in brain, kidney, liver and spleen tissues of mice to determine the viscera damage degree of restraint stress in mice. After the constraint is completed, get the plasma and liver tissue of mice. Test MDA content in liver tissue and activity of T-AOC and ALT in plasma of mice at different time to determine the best constraint time. The results show that there are extreme differences (P< 0.01) between constraint model group and blank control group on the MDA and T-AOC after constraint stress and constraint stress has the most obvious impact on the liver. When the constraint time is 18h, there are extreme difference between two groups on the MDA content in mice liver tissue and the activity of T-AOC and ALT in plasma (P<0.01). When the constraint time is 18h, the effect on causing the liver injury by oxidative stress is most obvious without no significant change as time extended, therefore constraint time of this the experimental model is 18 h.(3) Make constraint stress on mice for 18h to induce stressful liver injury on mice. Use NBT method, Reitman-Frankel method and TBA method to test activity of SOD, ALT and content of MDA in plasma. Adopt the GRIESS chemical method to test the NO content in liver tissue and use colorimetric method to test T-AOC level and activity of GSH-PX in liver tissue. After the constraint of 18h, the ALT activity and MDA content in plasma of mice in model group increase significantly. At the same time, it is also observed that SOD activity and T-AOC level in the liver tissue and plasma decrease obviously. Jujube peel red pigment can significantly constrain the rise of ALT activity and MDA content in plasma of the mice, improve the activities of SOD and GSH-PX in liver tissue and recover the T-AOC level in liver tissue. The results show that jujube peel red pigment has a protective effect on induced liver injury of constraint stress. |
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