| Part 1 Correlation between expression of immune molecules of erythrocyte and T lymphocyte subsets in bone and joint tuberculosis patientsObjective: To investigate the changes of immune molecules of erythrocyte and T lymphocyte subsets and their significances in patients with bone and joint tuberculosis. Methods: Venous blood was extracted from 110 patients with bone and joint tuberculosis and 104 healthy individuals(control group). Flow cytometry(FCM) was used to detect the immune molecules of erythrocyte, such as CD35, CD44, CD55, CD58 and CD59, as well as the concentration of T lymphocyte subsets, such as CD3+, CD4+ and CD8+. Results:(1) CD35, CD44, CD59, CD3+, CD4+ and CD4+/CD8+ in bone and joint tuberculosis group were significantly lower than those in control group(P<0.05), while CD8+ was higher than in control group(P<0.05).(2) There were positive correlations between CD35 and the percentage of CD3+ and CD4+ in the bone and joint tuberculosis group(P<0.05), while CD35 had no obvious correlation withCD8+ and CD4+/CD8+(P>0.05). Conclusion: In patients with bone and joint tuberculosis, erythrocyte immune function is low and turbulent. At the same time, T lymphocyte immune function is abnormal and disturbed. Erythrocyte immunity and T lymphocyte immunity possibly together take part in disease pathogenesis of bone and joint tuberculosis.Part 2 The association analysis of CR1 gene with bone and joint tuberculosisObjective: To investigate the association between single nucleotide polymorphisms(SNP) of erythrocyte CR1 and the susceptibility of bone and joint tuberculosis. Methods: The study was conducted in 110 cases with bone and joint tuberculosis and 104 healthy controls. The samples were collected for the detection of five SNPs(rs11118167 C/T, rs2274567 G/A, rs2296160 G/A, rs4844600 G/A and rs9429945 C/T) of CR1 gene by polymerase chain reaction-single base extension technique and DNA sequencing method. The genotypes and alleles frequency were analyzed by Statistic and the relationship between them and susceptibility to bone and joint tuberculosis was evaluated. Analysed the association between erythrocyte CR1 gene polymorphism and level of erythrocyte CR1 which detected before. Results:(1) The genotypes and alleles frequency of rs4844600 G/A and rs9429945 C/T were significantly different between bone and joint tuberculosis group and control group(P<0.05). When compared with noncarriers, the risk rates of developing bone and joint tuberculosis in carriers of rs4844600 G/A-GG genotype(χ2=8.026, OR=2.262, 95%CI=1.275-4.013) and G allele(χ2=5.054, OR=1.565, 95%CI=1.058-2.314), rs9429945 C/T-CC genotype(χ2=7.249, OR=2.067, 95%CI=1.197-3.568) and Callele(χ2=4.280, OR=1.550, 95%CI=1.022-2.352) were increased. The genotypes and alleles frequency of rs11118167 C/T, rs2274567 G/A and rs2296160 G/A were not significantly different between bone and joint tuberculosis group and control group(P>0.05).(2) The G-C haplotype of rs4844600 G/A and rs9429945 C/T was significantly associated with a increased risk of bone and joint tuberculosis(OR=1.541, 95%CI=1.042-2.280); The A-T haplotype was significantly associated with a decreased risk of bone and joint tuberculosis(OR=0.620, 95%CI=0.407-0.943).(3) In the bone and joint tuberculosis group, the rs11118167 C/T, rs2274567 G/A, rs2296160 G/A, rs4844600 G/A and rs9429945 C/T polymorphisms had no significant correlation with the level of CR1(P>0.05). In the control group, the CR1 gene rs11118167 C/T-TT genotype, rs2274567 G/A-AA genotype and rs2296160 G/A-AA genotype were associated with increased CR1 level(P < 0.05), rs4844600 G/A and rs9429945 C/T polymorphisms had no significant correlation with the level of CR1(P > 0.05). Conclusion:(1) CR1 gene rs4844600 G/A polymorphism is associated with the incidence of bone and joint tuberculosis, GG genotype and G allele are risk factors for bone and joint tuberculosis.(2) CR1 gene rs9429945 C/T polymorphism is associated with the incidence of bone and joint tuberculosis, CC genotype and C allele are risk factors for bone and joint tuberculosis.(3) CR1 gene rs11118167 C/T, rs2274567 G/A and rs2296160 G/A polymorphisms have no association with bone and joint tuberculosis susceptibility.(4) The haplotype G-C carrier of rs4844600 G/A and rs9429945 C/T carry a higher risk of bone and joint tuberculosis, the haplotype A-T carrier of rs4844600 G/A and rs9429945 C/T carry a lower risk of bone and joint tuberculosis.(5) The single nucleotide polymorphisms of CR1 gene may have no significant correlation with the level of erythrocyte CR1 in bone and joint tuberculosis patients. |
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