| Objective:To evaluate the contamination status of Vibrio parahaemolyticus(Vp) in aquatic products from Guiyang markets and analyze the toxic genes and antimicrobial resistance of the isolated strains.Methods:A total of 200 aquatic products were randomly collected in different food markets of Guiyang. Vp in aquatic products were identified according to national standard detection method GB 4789.7-2013. The toxic genes, tdh and trh, were detected by PCR method. In addition, the drug resistance tests of the isolated Vp strains were also performed via VITEK 2.Results:36 of 200 samples(18%) were identified Vp positive, which could be evenly separated into 5 serotypes, namely O1(6/36), O2(12/36), O3(12/36), O5(3/36),O10(4/36), with no predominant serotype being found. According to PCR results, 1Vp was tdh positive, all 36 strains were trh negative. One strain showed multi-drug resistance. 24 trains(66.67%) were resistant to ampicillin. All the strains showed sensitivity to ampicillin/sulbactam, piperacillin, piperacillin/tazobactam, cefotetan,ceftriaxone, cefepime, aztreonam, imipenem, meropenem, amikacin, gentamycin,tobramycin, ciprofloxacin, ofloxacin, nitrofurantoin and compound sulfamethoxazole.Conclusion:Part of the aquatic products in Guiyang were contaminated by Vp. And the isolated strains showed antimicrobial resistance. It is necessary to strengthen health supervision of aquatic products and management of antibiotics’ application in both aquaculture and hospital. Meanwhile, we should carry out more food safety education, go deep into the study and analyzing of Vp in aquatic products of our province, to provide data and techniques for disease prevention and government surveillance. |
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