Improving Method Of Trans-gastroduodenal Artery Reverse For Hepatic Artery Catheterization In Rat

Objective:To evaluate the value and feasibility of the trans-gastroduodenal reverse artery catheterization of hepatic artery catheterization in the hepatomagraft rat model. Methods: 120 adult health clean level of Wistar rats were randomly divided into group A,B,C and D, 30 rats in each group. The group A and B were the pre experimental group. The modified method and the traditional method were used for the hepatic artery catheterization.The rat lilver tumor model is planted in group C and D by the puncture method.Group C and D were treated with the improved method and the traditional method for the treatment of hepatic artery angiography and embolization respectively. The success rate of intubation, the success rate of rat liver cancer model, the change of tumor volume, the cause of death, the mortality of the rats in each group were statistically analyzed by two methods.Results: The intubation success rate of group A was 90.0%(27 / 30), and group B was 63.3%(19 / 30). the intubation success rate of group was significantly higher than that of group B(?2=4.565, P = 0.033). Tumor implantation success rate was 100% in the group C and D.Before the treatment,CT show the low density shadow in the left hepatic lobe without ascites and metastasis,and the edge is smooth.The success rate of intubation in group C was 93.3%(28/30) and 70%(21/30) in D group. The cause of death was vascular damage, liver injury and anesthesia accident.The overall success rate in C group was significantly higher than that of D group(?2=4.007, P=0.045). We can find no different of the average tumor volume before and after transcatheter arterial chemoembolization In group C and D(P>0.05). The 10 day mortality rate of group D was significantly higher than that of group C after operation(42.86% vs 10.71%, ?2=5.079/P=0.024).Conclusion: The modified rout for catheterization of hepatic arterial by gastroduodenal artery in rats is easy to operate successfully and has few complications and low mortality, It is worth continuing to study and try in the relevant experiment.