| Objective: To explore whether fat transplantation can improve the symptoms of the mouse model of Atopic Dermatitis(AD) induced by dinitrochlorobenzene(DNCB) as well as its possible mechanism, laying theoretical foundations for searching for new methods to treat atopic dermatitis, thus providing a more reliable basis for further clinical application.Methods: Seventy BALB/c mice were randomly divided into three groups: 50 mice for the experimental group(model group), 10 for the control group and 10 for the fat donor group. Applied DNCB on mice for the AD model, applied the same amount of acetone on the mice in the control group, then made the 20 mice randomly and averagely selected from the experimental group and the control group dead after establishing the model, finally confirmed successful establishment of the mouse AD model through pathology of tissues and the level of serum and cell factors related to skin lesion. The left mice in experimental group were averagely divided into four groups at random: fat transplantation group, pseudo surgery group, positive medication control group and spontaneously-recovered model group. Two weeks later, we got blood sample and the whole skin lesions from the mice after having them dead,then observed the pathology of skin tissues of each group with HE dyeing;detected the level of Ig E & IL-4 in blood serum by ELISA; quantitatively detected the expression level of L-10, IL-17 and IFN-γ in the skin lesions by PCR(RT-PCR).Results: Phenomena such as erythema, exudation, anabrosis and obvious scabs showed up on the back of all mice in the model group which were the typical clinical symptoms of atopic dermatitis; only skin conditions of mice with acetone application from the control group showed no overt difference from the beginning, as the pathology and the level of these cell factors were in line with reports of relevant documents and papers, the mouse AD model had been successfully established.In two weeks after fat transplantation, it was observed that horny layers of the fat transplantation group and the medication group were significantly thinner than that of the model group, and the thickness of epidermis of the former two groups got normal while no overt change happened to the pseudo surgery group and the spontaneously-recovered model group; besides, the levels of Ig E & IL-4 in blood serum detected via ELISA in the fat transplantation group and the medication group obviously dropped down compared to that of the model group, statistical significance manifested itself for p<0.05(t=11.322、10.403、5.783、6.358);there was no distinct decline here for the pseudo surgery group and the spontaneously-recovered model group compared to the model group(t=1.178、1.839、0.822、1.540,p>0.05). The expression levels of L-10& IL-17 detected by RT-PCR in skin tissues of both the fat transplantation group and the medication group were clearly lower than that of the model group, the level of IFN-γ for the two groups higher than that of the latter,statistical significance manifested itself for p<0.05(t=5.809、7.328、-5.071、2.585、3.713、-3.051); no overt change happened to the pseudo surgery group and the spontaneously-recovered model group compared to the model group(t=1.497、2.017、1.248、0.982、-0.894、-0.494,p>0.05).Conclusion: 1. DNCB-sensitized BALB/c mouse model shared similarity with symptoms of human’s atopic dermatitis; 2. After fat transplantation,levels of Ig E & IL-4 in blood serum of the mouse AD model dramatically dropping, the expression levels of L-10 & IL-17 in skin lesions decreasing, production of IFN-γ being improved, symptoms of skin lesion being eased and recovery of skin lesions of the mouse model being accelerated; 3. Fat transplantation could be adjusting and controlling the imbalance of Th1/Th2 and Treg/Th17 cells to reach a therapeutic function. |
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