| Objective:To explore the relationship between X-ray repair cross complementary gene 4 (XRCC4) rs6869366 and telomerase reverse transcriptase (TERT) gene rs2736098 single nucleotide polymorphism and bladder cancer susceptibility in Guangxi population.Method:With a case-control study method,201 cases of bladder cancer were selected as the case group, and 200 healthy people were collected as control group in Guangxi. PCR-RFLP method was used to detect the XRCC4 gene rs6869366 sites and TERT gene rs2736098 sites genotypes, and verify the results of genotyping by gene sequencing method. Using SRSS17.0 for statistical analysis. Between the two groups of genotype distribution, smoking status, gender, ethnic differences with chi-square test. With unconditional logistic regression to calculate the OR and 95% CI, assess XRCC4, TERT gene polymorphisms and risk of bladder cancerResults:1.There were no difference in age, gender and ethnic distribution between the two groups (P>0.05).The goodness-of-fit chi-square test shows that the control rs6869366 and rs2736098 genotype distribution conforms to Hardy Weinberg equilibrium, that controls the crowd with a group representative (P>0.05)2. TT rs6869366 genotype in case group was 170 cases, accounting for 85.1%, GT genotype was 30 cases, accounting for 14.9%; control group TT rs6869366 genotype was 173 cases, accounting for 87.1%, GT genotype was 27 cases, accounting for 12.9%, There was no difference in genotype frequency distribution between the two groups (P>0.05). The frequencies of T and G in the case group were 92.5% and 7.5% respectively, and 6.4% and 93.6% in the control group, there was no significant difference in the allele frequency distribution between the case group and the control group (P>0.05). Referring to the TT genotype with logistic regression analysis showed that, XRCC4 rs6869366 polymorphism was not associated with the risk of bladder cancer (OR=1.39,95%CI:0.39-4.97, P>0.05). According to the age, sex, ethnicity, smoking stratified analysis,show that XRCC4 rs6869366 polymorphism is not associated with susceptibility to bladder cancer. To further analyze the relationship between the rs6869366 XRCC4 polymorphism and the pathological stage and grading of bladder cancer. The results showed that rs6869366 XRCC4 gene polymorphism was not related to the pathological stage and grade of bladder cancer(P>0.05)3. Case group rs2736098 GG genotype for 58 cases, accounted for 28.9%, GA genotype of 95 cases, accounting for 47.3%, AA genotype for 48 cases, accounting for 23.8%; Control group rs2736098 GG genotype of 80 cases, accounting for 41.5%, GA genotype of 88 cases, accounting for 42.5%, AA genotype for 32 cases, accounted for 16.0%, there has obvious differences between the two groups.The proportion of G and A in the case group were 52.5% and 47.5% respectively, and the control group was 62% and 38%, respectively, and the two groups had significant difference. The non conditional logistic regression analysis showed that with AA genotype had an increased risk of bladder cancer compared with the GG genotype (OR=2.069,95% CI: 1.181-3.624, P<0.05). Further stratified analysis found that, in men (OR= 1.84,95%CI:1.15-2.94, P=0.011),older than 60 years old (OR=2.13,95%CI:1.23-4.00, P=0.008)、smokers (OR=2.00,95% CI:1.15-3.463,P=0.014), the risk of bladder cancer was significantly increased in individuals carrying the mutation genotype (GA+AA). There was no significant difference in the pathological staging and grading of bladder cancer (P>0.05).Conclusion:In the guangxi region,the study show that TERT rs2736098 polymorphism is associated with bladder cancer susceptibility, carrying rs2736098 AA genotype individual risk of bladder cancer is 2.069 times of the GG genotype; no found XRCC4 polymorphism rs6869366 sexual and bladder cancer predisposition. Further analysis did not find rs6869366 and rs2736098 with bladder cancer pathological stage and grade. |
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