The Association Of XRCC4 Single Nucleotide Polymorphism With Colorectal Carcinogenesis

Aim:to investigate the association of XRCC4 polymorphic variants at G-1394T (rs6869366) with colorectal cancer susceptibility.Methods:In this hospital based case control study,the association of XRCC4 polymorphism with colorectal cancer risk in Chinese people was investigated.In total, the peripheral blood of 159 colorectal cancer patients that admitted to our hospital from 2002 to 2008 was collected,and 110 healthy patients matched with age and gender were selected.The genomic DNA of the patients and controls was extracted and the 300bp target DNA was amplified,the product was then digested with HinⅡrestriction enzyme, followed by agarose electrophoresis to identify the genotype.Results:We found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female(p＜0.05).Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T(OR 0.098).While in overall population or male,there was no significant difference of distribution between the colorectal cancer and control groups.Conclusion:Our findings suggested that the G allele of XRCC4 G-1394T was associated with decreased risk of colorectal cancer susceptibility in female and shed new light on the genetic aspect of colorectal carcinogenesis.