Detection Of CK7mRNA And CXCL12 In Peripheral Blood Of Patients With Ovarian Cancer And Their Clinical Significance

Objectives:The purpose of this study was to investigate the detection of cytokeratin 7 mRNA and CXCL12 in peripheral blood of patients with epithelial ovarian cancer and to analysis of the value of both in the diagnosis of epithelial ovarian cancer.And discuss the relationship between cytokeratin 7,CXCL12 with the histological grade, pathological stage and retroperitoneal lymph node metastasis of epithelial ovarian cancer.In order to provide the basis for the evaluation of the patients’ condition before operation and a new idea of the clinical diagnosis and treatment for epithelial ovarian cancer.Methods:We collected peripheral blood samples from 44 patients of hospitalized patients with epithelial ovarian cancer between July 2012 and May 2014 in Third Affiliated Hospital of Kunming Medical University as the experimental group. And 10 patients of hospitalized patients with epithelial benign ovarian tumor as the benign group and 13 healthy women as control group. Real-time fluorescence quantitative reverse transcription polymerase chain reaction (qPCR) was used to detect expression level of CK7mRNA in peripheral blood. Enzyme-Linked Immunosorbent Assay technique was used to determine the content of CXCL12 in peripheral blood.Results: 1.The CK7mRNA expression level in the experimental group was 8.098±2.601, the benign group was 7.271±1.300 and the control group was 6.280±1.210, the difference was statistically significant (p=0.039).The difference between the experimental group and the control group was statistically significant (p=0.013), there was no significant difference in the expression level between the benign group and experimental group, control group(p>0.05). CXCL12 concentration in peripheral blood of the experimental group was 3.232±0.096, the benign group was 3.167± 0.191 and the control group was 2.741±0.232, the difference was statistically significant (p<0.001). there was significant difference between the control group and experimental group, benign group (p<0.001), there was no significant difference between the benign group and experimental group (p>0.05).2. There was a correlation between the CK7mRNA expression level in peripheral blood of patients with epithelial ovarian cancer and the retroperitoneal lymph node metastasis (P<0.05) and there was no significant difference with age,pathological stage, histological grade, abdominal pelvic implant (P>0.05). The determination of CXCL12 concentration has no significant difference with age, histological grade, abdominal pelvic implant (P>0.05), but was significantly correlated with pathological stage and retroperitoneal lymph node metastasis (P<0.05).3. There was no correlation between CK7mRNA expression level and CXCL12 expression in peripheral blood of patients with epithelial ovarian cancer (r=0.222; p=0.148).4. Combined detection of CK7mRNA and CXCL12 was significantly different with the retroperitoneal lymph node metastasis (P<0.05), but there was no significant difference with pathological stage, histological grade, abdominal pelvic implant (P>0.05).Conclusions:1. The expression level of CK7mRNA and CXCL12 in peripheral blood of patients with epithelial ovarian cancer was obviously higher than patients with epithelial benign ovarian and healthy female, both of them may be involved in the occurrence and development epithelial ovarian cancer.2. By detecting CK7mRNA expression level and CXCL12 concentration in peripheral blood can be used as a reference index for preoperative assessment and predicting the lymph node metastasis in the patients with ovarian cancer.