| Objectives:To investigate the apoptosis effect of gastric cancer SGC-7901 treated by curcumol, inhibition ratio of proliferation of gastric cancer SGC-7901 cells, morphology changing, DNA changing and expression of important apoptosis protein Bcl-2 were detected. The results will be helpful for elucidating mechanism of curcumol anticancer, offering experimental data for clinic applying.Object:Human gastric cancer cells line: SGC-7901.Methods:Curcumol was dissolved in dehydrated alcohol and initial concentration of curcumol was 5mg/mL. Group A,B,C and D as experimental groups were set up .The curcumol concentrations were set as follows: 12.5,25,50,100μg/mL. Group E was control group, containing 1% dehydrated alcohol. After seeded for 24, 48 and 72h, inhibition ratios of SGC-7901 cells proliferation were detected by MTT assay and alamar blue assay, and morphology changings of apoptosis were detected by AO/EB fluorescent staining. After seeded for 24 and 48h, apoptosis ratios were detected by FCM and the expressions of bcl-2 were detected by Western Blot.ResuLts:1. 12.5-100μg/mL curcumol could restrain proliferation of SGC-7901 after treated for 24, 48 and 72h detected through MTT assay. With the increase of curcumol concentration and the incubation time, the inhibition was reinforced. Comparing to control group, there... |
