| Object:To research the influence of Chaihushugan (CHSG) Powder on the gastric emptying, gastrointestinal hormone and small intestine microstructure in the model rats with liver depression syndrome. With the perspective of gastrointestinal hormone and ICC cell, the pharmacological effects and mechanism of CHSG Powder are revealed for the gastrointestinal motility in model rats. Meanwhile, the whole regulating advantages of Chinese medicine to treat spleen diseases through smoothing the liver is uncovered.Methods:1. The establishment of rat model with liver depression syndrome:the chronic unpredicted mild stress (CUMS) was applied to establish the model for three weeks. And the general conditions, weight, volume of food-intake and motionless time of forced-swimming were measured before and after modeling for the evaluation of modeling effects.2. The influence of CHSG Powder on serum levels of motilin (MOT), Somatostatin (SS) and gastric emptying in the rat with liver depression syndrome:after modeling, medication with 10 ml·kg-1ig per day was administrated for two weeks according to dosage of each group. The concentrations of medication in the high-dosage and low-dosage CHSG Powder groups were 21.0 g·kg-1 and 10.5 g·kg-1 respectively; the concentration of medication in domperidone group was 10 mg·kg-1; isovolumetric normal saline was prescribed for the blank control group and modeling group. Before and after modeling as well as after administrating, ELISA was applied to measure the MOT and SS level of rat serum in each group. And the phenol-red-method was used to measure the gastric emptying rate.3. The influence of CHSG Powder on ICC-DMPs in model rat:small intestine tissue of 8 cm with 1cm distance to pylorus was selected from the blank control group, the modeling group and the administration group. And the tissue was incised along the mesentery brim to remove the contents and then put the tissue into the stationary fluid. After station, the electron microscopic section was prepared routinely. Later, electron microscope was applied to observe the ultra structure of ICC-DMPs.Results:Experiment 1:General condition of rats in blank control group almost steady before and after modeling. The rats’hair in modeling group turned gradually into a mess after modeling. And there were fewer activities with the manifestations of lassitude, fatigue and doziness. Meanwhile, the stable mood of rates was changed into the irritable and then the depressed condition. In addition, the food intake of rats was gradually reduced, and the stool of them was gradually soft and even loose. The motionless time of forced-swimming test of modeling group was significantly increased than that of the blank control group (p<0.01). On the weight aspect, the rats’weight of blank control group, while that of modeling group manifested a slower growth or even a negative growth (p<0.01). And the weight change was not relevant with gender. When it comes to gastrointestinal hormone, the MOT secretion was significantly reduced (P<0.05). And SS secretion was obviously increased (P<0.05) before and after modeling in each modeling group.Experiment 2:Model rats with liver depression syndrome were succeeding since the results of experiment two were basically similar to experiment 1 comparing before and after modeling. On the aspect of food intake after medication, each administration group was on the same level with that of blank control group (P>0.05). When compared with blank control group, the mean daily food intake of every administration groups was on the same level (P>0.05), but much higher than that of the modeling group (P<0.05), especially in domperidone group (P<0.01).For the weight, the rats’ weight of administration group was rebounded compared with modeling group (P<0.05), especially in domperidone group. In the two groups treated with CHSG Powder, the rats’ weight of the low-dosage group recovered in a lower speed than the high-dosage group. For the behavioral changes, motionless time of rats in modeling group was longer than blank control group (P<0.05), while that of high and low dosage of CHSG Powder groups which was no obvious significance was less than that of modeling and domperidone group (P<0.05). For the gastric empting rate, the blank control group and administration groups were higher than modeling group (P<0.05). and low dosage of CHSG Powder was most obviously (P<0.01), the significance of each administration group was not obvious (P>0.05). When it comes to gastrointestinal hormone level, MOT secretion of modeling group recovered but still generally lower than that of blank control group which was of no significance statistically. The MOT level of administration groups was higher than the blank control group and modeling group (P<0.05). In addition, the serum SS level of rats in domperidone group decreased than that of blank control group(P<0.05). And the serum SS level of rats in administration group decreased obviously than that of modeling group (P<0.01)Experiment 3:The total number of ICC-DMPs with the gap junction of smooth muscle cell and peripheral nerve fibers in modeling group was reduced. The ICC-DMPs was polygon shape with less cytoplasm, expanded mitochondria with fractured cristae, expanded rough endoplasmic reticulum and abundant euchromatin in cell nucleus. After the treatment of high-dose CHSG Powder, the number of ICC-DMPs was obviously increasing with shuttle shape, less cytoplasm, abundant intra-cytoplasm glycogenosome, part expanded endoplasmic reticulum, rod-shaped cell nucleus, abundant heterochromatin and part swelling mitochondria less than the modeling group obviously. The effect of low-dose CHSG Powder for the structure improvement and quantity of ICC-DMPs was not better than the high-dose group, but there was still good outcomes. In addition, the structure improvement of ICC-DMPs in domperidone group was not obvious.Conclusions:1. The effect of receiving CHSG Powder can improve the food intake and weight as well as shorten the motionless time of forced-swimming for model rats with liver depression syndrome.2. The gastrointestinal motility mechanism of model rats with liver depression syndrome improved by CHSG Powder can regulate the MOT and SS level upward in blood.3. The recovering of ICC-DMPs quantity and shape in the model rats can be promoted by CHSG Powder in a certain extent. |
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