| Objective:To study the expression of Aurora-A in renal cell carcinoma and cancer side normal tissue and its clinical significance, and to evaluate the possibility of Aurora-A as a potential bio-marker of renal cell carcinoma prognosis and a new target for renal cell carcinoma targeted therapy.Methods:23 cases of Renal cell carcinoma specimens and Adjacent normal kidney tissues were collected in our hospital Urology department. respectively using immunohistochemistry and Quantitative Real-time PCR to detect Aurora-A expression in 23 cases of surgical resection for renal cell carcinoma patients with renal cell carcinoma and normal renal tissue. Respectively using Paired samples t-test and Chi-square test from SPSS 17.0 to analysis expression level differences of Aurora-A protein and mRNA in Renal cell carcinoma and Corresponding adjacent normal renal tissues. At the same time, using Fisher probability method to analysis clinical pathological features relationship between Aurora-A protein expression and renal cell carcinoma. Using Independent samples t-test and one-way ANOVA to analysis clinical pathological features relationship between Aurora-A mRNA expression and Renal cell carcinoma, inspection level is a=0.05.Results:1. There was a positive Aurora-A protein expression in renal cell carcino ma and normal renal tissues adjacent to carcinoma, the high expression of Aurora-A protein in renal cell carcinoma(RCC) was 65.22%, significantly higher than that of nor mal renal tissues adjacent to cancer 30.43% (X2=5.567, P=0.038<0.05);2. Aurora-A protein high expression rate of patients with renal clear cell carcino ma Ⅰ、Ⅱ、Ⅲ(clinical stage) were 28.6%,66.7% and 100%, respectively, with significa nt difference (P=0.024< 0.05);3. Aurora-A protein high expressions rate in renal clear cell carcinoma (high, med ium and low) were 40.0%,57.1% and 75.0%. Aurora-A protein high expression rate wi 11 increase with reducing of the differentiation degree, but differentiation was not statis tically significant (P=0.537>0.05);4. Aurora-A protein and mRNA expression in renal clear cell carcinoma is not significantly correlated with patients’ sex, age, size of tumors (P>0.05);5. Aurora-A mRNA expression in renal clear cell carcinoma was significantly higher than that in normal renal tissues adjacent to carcinoma (2.1283±0.6699 VS1.0561±0.3880), the difference was statistically significant (t=7.539, P<0.05)6. Aurora-A mRNA relatively expressions of patients with Renal clear cell carcinoma Ⅰ、 Ⅱ、Ⅲ were 1.417±0.555,2.176±0.165 and 2.825±0.466, expressions will increase with clinical stage increasing. The difference was statistically significant (F=18.006, P=0.001< 0.05);7. Aurora-A mRNA relatively expressions in Renal clear cell carcinoma (high, medium and low) were 1.930±0.472,2.159±0.718 and 2.263±0.798. The difference was not statistically significant (F=0.345, P=0.713>0.05)Conclusions:Aurora-A in renal cell carcinoma showed higher expression than that in adjacent normal renal tissue, and Aurora-A protein and mRNA expression shows correlation to tumor clinical stage. Aurora-A is closely related to renal cell carcinoma occurrence and development; Aurora-A may be potential bio-markers for the clinical prognosis judgment of renal cell carcinoma, and become a new target for the treatment. |
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