Expression And Clinical Significance Of IL-36γ In Squamous-cell Carcinoma Patients

Objective:To investigate the expression and clinical significance of cytokine IL-36γ in squamous-cell carcinoma(SCC).Methods:To collect 42 tumor tissues and adjacent normal tissues from the same patient with definite pathological diagnosis of SCC in The Third Affiliated Hospital of Soochow University.Real-time quantitative PCR was performed to detect the mRNA expression of IL-36γ in 42 pairs of cancerous tissues.In addition,immuno-histochemistry was used to evaluate the expression and location of IL-36γ in selected 47 cases,to analyze the correlation of IL-36γ expression with clinic pathological variables,tumor size,clinical stage and overall survival.Results:The expression of IL-36γ mRNA in cancer tissues washigher than that in adjacent normal tissues(p<0.05).The expression of IL-36γ mRNA in early stage cancer was higher than that in advanced cancer(p<0.05).The immunohistochemical results showed that IL-36γ was expressed in bronchial epithelial cells,chondrocytes and vascular endothelial cells.The expression of IL-36γ in cancer tissues was significantly higher than that in adjacent normal tissues(p<0.0001).Besides,the expression of IL-36γwas associated with tumor size and pathological TNM stage.No significant correlation was found between the IL-36-γ expression and age,differentiation status,lymph node metastasis and overall survival time of patients.Conclusion:The expression of IL-36γ was higher in the early stage of squamous-cell carcinoma than that in advanced cancer,and the expression of IL-36γ in tumor tissues was significantly higher than that in adjacent normal tissues.It is suggested that IL-36γ may play an important role in the development of SCC and the treatment of immune intervention.