Detection And Diagnostic Evaluation Of Prostatic Exosomal Protein In Chronic Prostatitis Patients

Objective: Chronic prostatitis(CP) is one of the most common urologic diseases in young males. The etiology and pathogenesis is still a complex. The diagnosis of NIH-Ⅲaprostatitis mainly based on patient’s subjective experience, because of laboratory examination methods are limited. We detected urine prostatic exosomal protein(PSEP) to evaluate its clinical potentialas a diagnostic marker of CP.Methods:A totle of 59 cases of NIH- Ⅲ aprostatitispatiensand 96 cases of controls wereenrolled from May 2014 to June 2015, in the first people’s Hospital of Taicang,Jiangsu Province.Urine samples were collected for test,using ELISA methods for the detection of PSEP. PSEP levels between the prostatitis group and control group were compared. A ROC curve was developed for calculating the sensitivity, specificity and calculating the optimum Youden index, so as to find cut-off value of PSEP.Results: A total of 59 cases of prostatitis group, 96 cases in the control group. Both groups median age is 30(26,39) and 29(23,37), no significant difference in age distribution. The data of urine PSEP showed a non-normal distribution. The median PSEP ofprostatitis Group is 0.966(0.296,3.294)ng/ml, which is higher than the control group0.193(0,0.734) ng/ml, using Mann Whitney u test, p<0.01, and the difference was significant. Using SPSS20.0 statistical software to develop a ROC curve fitting PSEP results.The area under the curve is 0.740.The value of 0.495ng/ml, corresponds to the maximum Youden index,was selected toserve as the role of diagnosticcutoff value, leading to a diagnostic sensitivity of 71.2%, and a specificity of 69.8%.Conclusion:Urine PSEP as a diagnostic marker of NIH- Ⅲ a prostatitis, shows a medium ability in CP diagnosing, but a higher conveinenceapplying in clinic.Establishment of PESP detection methods, could possiblycompensate for the clinical diagnosis of chronic prostatitis, and may provide a novel way for the diagnosis of thechronic prostatitis. Based onthesimple, non-invasive, pain-free features of PSEP, better clinical application is convienced.