Research On The Effects Of Titanium Particles Intervention Of MLO-Y4 Cells To The MC3T3-E1 Cells Functions

Background:Previous scholars mostly study osteoblast and osteoclast bone dissolve mechanism, in fact accounted for over 90% of the bone tissue,osteocytes and bone has a close relationship between dissolved. The present study showed that osteocytes is not relatively stationary and dormancy,osteocytes can be through the bone on the surface of the tubular and dendrites and osteocytes communicate with each other information,and at the center of bone reconstruction the sponsor of the regulating cell and bone reconstruction.Have reported literature has a two-way adjustment role in osteocytes SOST(sclerosteosis) gene and the protein is serve as a bridge between osteocytes and osteoblast or osteoclast, however,the study of titanium particles intervention of osteocytes to osteoblasts functions is relatively small.Objective: to study the titanium particles on bone cell proliferation 、apoptosis exists,research on the effects of Titanium particles intervention of MLO-Y4 cells to the MC3T3-E1 cells functions, further understanding of osteocytes around the implant bone dissolve play a role in the process.Methods : cultured MLO-Y4 osteocytes were exposed to different concentrations of titanium(Ti) particles,cell viability was measured using the Cell Counting Kit-8(CCK-8) assay,apoptosis of MLO-Y4 cells was evaluated by flow cytometry,Real-time PCR quantification of mRNA expression of SOST,at the same time with Western Blot detection sclerosteosis protein expression levels.MC3T3-E1 cells culture with MLO-Y4 cells exposed todifferent concentrations of titanium(Ti) particles in vitro,in order to detection of osteoblast osteogenetic activity.Results:Our results showed that Ti particles inhibited cell viability of MLO-Y4 osteocytes in a dose-dependent manner. Incubation with Ti particles caused apoptosis of MLO-Y4 cells.Treatment with Ti particles significantly increased expression of the osteocytic marker SOST/sclerostin. Furthermore,treatment of MLO-Y4 cells with Ti particles produced a dose-dependent decrease in ALP activity and decreased mineralization of MC3T3-E1 cells through direct cell-cell contact.Conclusion:Titanium particles damage osteocytes and inhibit osteoblast differentiation.