The Protection Effect Of Sarcandra Glabra And Irisquinone On Radiation-Induced Lung Injury In Rat

Objective:To evaluate the protective effect of Sarcandra Glabra and Irisquinone againist radiation-induced lung injury (RILI) in SD rat.Methods:One hundred and tweenty SD rat were randomly divided into four groups:normal group (group A), radiation group (group B), radiation plus Sarcandra Glabra (group C), Radiation plus Irisquinone group (group D), and each group has thirty rat. Before radiation, the same volume of normal saline solution was given daily in normal group (A), radiation group (group B); radiation plus Sarcandra Glabra (group C) were administered daily via gavage with Sarcandra Glabra (5g-kg-1·d-1).Radiation plus Irisquinone group (group D) were administered daily via gavage with Irisquinone (31mg·kg-1·d-1) for one week, after irradiation, continued administration until the lung tissue was moved. The model of RILI in SD rats in radiation group (group B), radiation plus Sarcandra Glabra (group C), Radiation plus Irisquinone group (group D), were established by irradiating right whole chest with a single dose of 20 Gy of 60Coy therapeutic machine,without the rats in normal group (group A).Rats were sacrificed from the four groups at week 1,4,8,12and 24 post-irradiation,the pathological changes of the lung stained with HE/Masson were observed with a light microscope; the content of hydroxyproline in lung tissue were examined by UV spectrophotometer; the tumor necrosis factor α(TNF-α), interleukin-1β(IL-1β) and transforming growth factor β1(TGF-β)in serum were examined by enzyme-linked immunosorbent assay.The expression of tumor necrosis factor α(TNF-α) and transforming growth factor β1(TGF-β1) in lung tissue were detected by immunohistochemical method.Results:At all time points, normal group (group A) showed that alveolar structure integrity, no exudation and edema of inflammatory cells.At the time point of 1、4、8week, normal group (group A) showed that there was no inflammatory cell exudation and edema. At the time point of 1、4、8week, radiation group (group B) showed that there were lymphocytes and neutrophils and macrophages focal inflammatory cells infiltration, alveolar capillaries expansion generally, pulmonary interstitial hyperemia and edema, thickening of alveolar walls, alveolar interval gradually; At the time point of 12、24 week, thickening of alveolar walls are severe, alveolar cavity decreased significantly or even missing, bronchi and vessels, alveolar interval has a large number of fibroblasts hyperplasia formation of fibrosis. At the time point of 1、4、8、12 week,Radiation plus Sarcandra Glabra (group C), Radiation plus Irisquinone group (group D) also appeared at the same time points above pathological changes,but pathological examination showed that there was an obvious amelioration in pathological injury of lung tissue in group C and group D. But at the time point of 24 week, thickening of alveolar walls heavy, there was an obvious amelioration in pathological of pulmonary fibrosis in group D, but has no evident difference compared with group C.The expression level of TNF-α:at the time point of 1、4、8week, the serum levels of TNF-α in radiation group (group B) were significantly higher than that of group C and group D(P<0.05); at the time point of 12,24 weeks, there was significant difference compared with group D(P< 0.05),but there was not significant difference compared with group C (P> 0.05). The expression level of IL-1β:at the time point of 1、4、8week, the serum levels of IL-1β in radiation group (group B) were significantly higher than that of group C and group D(P<0.05); at the time point of 12,24 weeks, there was significant difference compared with group D(P< 0.05),but there was not significant difference compared with group C (P> 0.05).The expression level of TGF-β1 and the content of hydroxyproline in lung tissue:at the time point of 4-.8week, the serum levels of TGF-β1 and the content of hydroxyproline in lung tissue in radiation group (group B) were significantly higher than that of group C and group D(P<0.05); at the time point of 12,24 weeks, there was significant difference compared with group D(P< 0.05),but there was not significant difference compared with group C (P> 0.05).The expression of tumor necrosis factor a (TNF-a) in lung tissue:at the time point of 4、8、12 week, the expression of tumor necrosis factor a (TNF-a) in lung tissue of radiation group (group B) were significantly higher than that of group C and group D(P<0.05);at the time point of 24 weeks, there was significant difference compared with group D(P< 0.05),but there was not significant difference compared with group C (P> 0.05).The expression of transforming growth factor β(TGF-β1) in lung tissue:at the time point of 4、8、12 week,the expression of transforming growth factor β1 in lung tissue of radiation group (group B) were significantly higher than that of group C and group D(P<0.05); at the time point of 24 weeks, there was significant difference compared with group D(P< 0.05),but there was not significant difference compared with group C (P> 0.05).Conclusion:Sarcandra Glabra and Irisquinone could alleviate acute radiation-induced lung injury by reducing the expression of TNF-α、IL-1β、 TGF-β1 in serum and the expression of TNF-α、TGF-β1 in lung tissue, there was not significant difference between each other in the protective effect on the acute radiation-induced lung injury. Irisquinone can reduce radiation pneumonitis, but also can reduce late radiation-induced pulmonary fibrosis. Sarcandra Glabra can reduce radiation pneumonitis, but the protective effect on the late radiation-induced pulmonary fibrosis was not obvious.