The Study On Expressions And Functions Of MiR-150-5p In Chordoma

Objective: To study the effect of miRNA-150-5p on the capacities of proliferation and invasion and the radiosensitivity of chordoma U-CH1 cells, the expression level of miRNA-150-5p was regulated.Methods: Using micro-array analysis our previous data revealed that the expression level of miRNA-150-5p in chordoma was obviously higher than that in nucleus pulposus. The expression levels of miRNA-150-5p in 25 specimens of chordoma and 8 nucleus pulposus samples were detected by qRT-PCR. miR-150-5p was over-expressed by miR-150-5p mimic while inhibited by miR-150-5p inhibitor in U-CH1 cells. The effect of miR-150-5p on proliferation and radiosensitivity was investigated by clonogenic assay, while the transwell migration assay was performed to determine the invasion ability in U-CH1 cells transfected with miR-150-5P mimic or inhibitor.Results:(1) The expression level of mi R-150-5p in chordoma was significantly higher than that in nucleus pulposus, which was in agreement with the results of micro-array analysis.(2) Clonogenic assay revealed that the level of miR-150-5p in U-CH1 cells was positively correlated with the capacity of proliferation.(3) Clonogenic assay also demonstrated that the radiosensitivity of U-CH1 cells was negatively correlated with miR-150-5p expression.(4) Transwell migration assay indicated that U-CH1 cells became much more invasive when transfected with miR-150-5p.Conclusions: The expression level of miR-150-5p in chordoma was considerable higher than that in nucleus pulposus. miR-150-5p over-expression could up-regulate the capacity of proliferation and invasion in U-CH1 cells, and make cells more radioresistant. However, miR-150-5p inhibition made U-CH1 cells less proliferative, less invasive and much more radiosensitive.