| ObjectiveThis research mainly uses the UPLC/Q-TOFMS tablet treatment of patients with psoriasis vulgaris before and after the change of the serum and urine metabolic state, and compared with healthy control group, to understand the patients with psoriasis vulgaris serum and urine of the basic characteristics of the metabolic profile, and to provide effective evidence from the level of metabolism in treatment set forth Yinxieling possible mechanism of psoriasis vulgaris, is Yinxieling treatment of psoriasis vulgaris.MethodsIn February 2014-September 2015 in guangdong hospital dermatology clinics and consistent with subject standards of psoriatic patients to silver crumbs spirit treatment. In (0 weeks and 12 weeks) before and after the treatment respectively to evaluate the clinical evaluation indexes, and coagulant pipe and clean urine sampling since morning fasting blood in patients with 5 ml and 10 ml of midway through the urine. The liquid mass spectrometry detection analysis before and after treatment in patients with the changes of serum and urine metabolic state, and find the difference, the endogenous metabolites, so as to screen out patients with psoriasis vulgaris and silver crumbs spirit change after treatment of potential biomarkers.ResultsThis study participants included 18 cases of patients with psoriasis vulgaris, at the same time in guangdong hospital of a medical center in healthy control group, a total of 32 cases with the treatment group there was no statistically significant difference in gender, age.This study uses a high-throughput, high sensitivity and accuracy of the UPLC/Q-TOFMS technology for serum and urine specimens collected injection detection and analysis, With the PCA, PLS-DA, OPLS-DA methods to establish model. Before and after patients with psoriasis vulgaris morning urine and serum metabolomics treatment compared with the healthy control group, the metabolic outline more clearly distinguish clearly; before treatment with serum and urine metabolic biomarkers healthy controls suggests that psoriasis vulgaris disease and lipids, vitamins, amino acids and coenzyme related; metabolic pathway before and after treatment serum lipid metabolism mainly in the main, considered related to lipid metabolism disorders may lead to psoriasis, many of whom triglyceride and phospholipid Metabolism; and morning urine before and after the treatment of metabolic biomarkers before treatment and health groups tend to be similar. According to pre-treatment with the first morning urine and serum of healthy control group of biomarkers, the presence of psoriasis with healthy metabolic differences, the metabolic disorder associated with lipids, amino acids, vitamins, etc.; metabolism in the serum of patients with morning urine the difference was ceramide(dl8:1/9Z-18:1) and ceramide(d18:1/20:0) belong to the phospholipid metabolism ceramide-like substances, are present after treatment decreased, reflecting the Yinxieling regulation, may be considered the key to the development of psoriasis and Yinxieling regulation, but require a lot of repeated experiments to prove it.ConclusionMetabolism of serum and urine with psoriasis vulgaris and that there was a significant difference between the healthy control group after treatment with silver crumbs spirit, its metabolic difference obvious change and after the treatment of metabolic data tend to be more healthy normal group; In metabonomics, silver crumbs spirit treatment has research value with psoriasis vulgaris, combined with related metabolic biomarkers, to further explore the mechanism of action of silver crumbs spirit on psoriasis and targets to provide powerful science basis. |
