Microcosmic Analysis And Molecular Mechanism Exploring Of The Implant-Bone Interface At Early Stage In Hyperlipidemic Rats

ObjectiveTo explore the micro-component and micro-morphology of implant-bone interface at early stage and to explore potential molecular mechanism related to Dishevelled-2 (DVL2) for early osseointegration of dental implants in hyperlipidemic rats.Methods(1).60 Wistar rats were divided into the experimental group and the control group. They were given a high-fat diet and a normal diet respectively.(2). After 12 weeks, titanium implants were planted into femora of hyperlipidemic and normal rats. Rats were executed at 1,3 and 5 days after implantation.(3). Hard tissue sections containing micro-screws at 1 and 5 days were made. The dynamic change for micro-morphology of implant-bone interfaceat early stage was analysed by microscope and SEM.(4). The dynamic change for micro-component of implant-bone interfaceat early stage was analysed by EDS.(5). RT-PCR and western blot were used to measure the mRNA expression and the protein expression of the DVL2 and phospho-DVL2 at 1,3 and 5 days respectivly.Results(1). The results of microscope indicated there were fewer osteoblasts (P< 0.05) but more osteoclasts (P<0.05) in the experimental group.(2). The results of SEM were similar with the results of optical microscope.(3). The ratio of Ca/P of the experimental group was lower (P< 0.05), however, the content of O was opposite (P< 0.05). Ti content gradually reduced from implants to bone, and there were not notable exceptions between the two group.(4). The mRNA expression of DVL2 in the experimental group was weaker (P< 0.05).(5).The expression of DVL2 and phospho-DVL2 protein in the experimental group was both lower (P< 0.05).ConclusionsHyperlipidemia interferes with the early osseointegration of implants may via inhibiting expression of DVL2 mRNA, DVL2 protein and phospho-DVL2 protein.