| ObjectiveTo test the expression of long noncoding RNA MEG3 in gastric cancer tissues and analyze the relationship between the expression level of lncRNA-MEG3 and the clinicopathological factors in patients with gastric cancer. In addition, we further studied weather the lncRNA-MEG3 can regulate the cell cycle of gastric cancer cells via vitro experiment and preliminary explore the molecular mechanisms.Methods1:qRT-PCR technology was used to determine the expression of lncRNA-MEG3 in the gastric cancer tissues and adjacent tissues. We further collected the personal data of the patients to analyze the association between the expression of lncRNA-MEG3 and the clinicopathological factors in human gastric cancers. We further detected the expression level of P53 mRNA in the gastric cancer tissues and adjacent tissues.2:In addition we also tested the expression level of lncRNA-MEG3 in gastric epithelial cells and gastric cell lines such as AG、BGC823、SGC79011、N87.3:qRT-PCR technology was also used to test the expression of lncRNA-MEG3 after the momentary transfection of pCDNA-MEG3 plasmid and blank plasmid into BGC823 cells using Lip2000. During the experiment, the GFP fluorescent protein was used to observe the efficiency of transfection. In addition, we used the PI dyes to detect the cell cycle of cells.4:Considering that P53 protein was predicted to be the downstream of lncRNA-MEG3, we further explored the variations of P53 protein in every group and attempted preliminary elaborating the molecular mechanisms of lncRNA-MEG3 regulating the cell cycle in gastric cancer.Result1:In our study, we found that the expression level of MEG3 in gastric cancer tissue were much lower than that of adjacent tissues. What’s more, the expression of lncRNA-MEG3 in patients with distant metastasis was significantly higher than that in other tissues. However, lncRNA-MEG3 expression level didn’t show significantly association with other clinicopathological factors such as gender, age, diameters. And the expression of p53 mRNA also showed the significant difference in gastric cancer tissues and adjacent tissues.2:We also found that the expression level of MEG3 in gastric cancer cell lines were much lower than that GES-1 cell line.3:Momentary transfection of pCDNA-MEG3 plasmid could significantly inhibit the cell cycle by the G1 arresting in the BGC823 cell line and no significant difference was displayed between the other two groups.4:What’s more, we also found the expression of P53 protein was upregulated by the pCDNA-MEG3 plasmid in a great extent.Conclusion1:Our study displayed that lncRNA-MEG3 was down expression in gastric cancer and related with distant metastasis.2:lncRNA-MEG3 could regulate the process of carcinogenesis by up regulating P53 protein and may serve as new biomarker and therapeutic target of gastric cancer in future. |
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