| Objective:Through ulinastatin content of the different stages of chronic kidney disease patients in peripheral blood and urine were measured to compare the UTI content, to analyze the relationship between UTI and the renal function and its protective effect on the kidney.Methods:Sixty clinically diagnosed as chronic kidney disease patients as the experimental group from the First Hospital of Jilin University between December 2015 to March 2016.According to Modification of Diet in Renal Disease formula to calculate glomerular filtration rate(GFR)and divide them into CKD 3,4,5 three groups of 20 people. Other clinical data and laboratory index were collected including the patients’ sex,age,weight, blood pressure, medical history,SCr,BUN,ALB,white blood cell count,hs-CRP,PTH.The healthy control group of 20 cases were selected from the First Hospital of Jilin University Medical Center.By ELISA method to detect ulinastatin content in peripheral blood and urine of each group,Analysis the relation between the UTI content in peripheral blood and urine of every group of chronic renal kidney disease and renal function by statistical methods, and to explore the factors that may affect its content changes and its protective effect on the kidney.Results:1.The serum creatinine levels and the CFR levels of chronic kidney disease patients in CKD3 、4、5group,serum creatinine levels were(182.94 ± 70.01) μmol / L,(352.89 ±114.09) μmol / L,(836.50 ± 245.44) μmol / L; GFR were(42.65 ± 9.37) ml / min /1.73 ㎡,(18.85 ± 3.01) ml / min / 1.73 ㎡, Three patients in 3 groups with serum creatinine levels was gradually increasing trend, GFR actually was a gradual downward trend, indicating that the level of serum creatinine inversely with changes in GFR, the higher creatinine, lower e GFR..2.The ulinastatin content in peripheral blood and urine of chronic kidney disease patientsand normal control group : CKD3 、 4 、 5group and normal control group,UTI levels in inperipheral blood were respectively(139.35-7.72 mg/L),(192.42 + 18.) mg/L,(198.75-29.08mg/L),(40.90-9.42 mg/L), levels in urine, were respectively(39.00-4.34 mg/L),(63.80-9.88mg/L),(67.14-15.05 mg/L),(2.21-0.18 mg/L). ulinastatin content of chronic kidney disease patients in peripheral blood and urine were obviously higher than normal control group, and ulinastatin content in blood and urine have the negative correlation to the glomerular filtration rate.3.Chronic kidney disease patients and normal control group content of ulinastatin in peripheral blood and urine : pearson correlation analysis showed ulinastatin in peripheral blood and urine of chronic kidney disease patients and normal control group were significantly correlated(r chronic kidney disease patients=0.982,P<0.001),(r normal control group=0.973,P<0.001).4.The laboratory index of chronic kidney disease patients in CKD3 、4、5group:There are significant differences among three groups in cteatinine,BUN,Hemoglobin, hs-CRP and PTH(P<0.01).There were no significant differences in serum albumin, white blood cell count(P>0.05).5.Univariate analysis showed that in patients with CKD, UTI in peripheral blood and hemoglobin was negatively correlated(r =-0.469, P <0.001), reacted positively correlated with parathyroid hormone and hs-CRP(r = 0.574,0.404, P <0.001, P = 0.001), has no correlation with albumin(r = 0.063, P = 0.634), UTI in urine and hemoglobin was negatively correlated(r =-0.443, P <0.001), reacted positively correlated with parathyroid hormone and hs-CRP(r = 0.587,0.367, P <0.001, P = 0.004), has no correlation with albumin(r = 0.064, P= 0.630).Multiple linear regression analysis showed that glomerular filtration rate was negatively correlated with UTI in both peripheral blood and urine(P <0.001, P = 0.008),parathyroid hormone was positively correlated with UTI in both peripheral blood and urine positively correlated(P = 0.019, P <0.001).Conclusions:1.In patients with chronic kidney disease, UTI content in peripheral blood and urine were significantly higher than normal,suggesting UTI correlated with renal function,UTI content in urine has positive correlation with TUI in the peripheral blood.2.Univariate analysis showed that in patients with CKD, UTI in peripheral blood and urine had negative correlation with hemoglobin, reacted positively correlated with parathyroid hormone and hs-CRP, had no correlation with albumin.3.Multiple linear regression analysis showed that the UTI content in peripheral blood and urine has negative correlation with glomerular filtration rate and has positive correlation with parathyroid hormone in patients with chronic kidney disease,suggesting that with the decline of renal function, the oxidative stress strengthened and trypsin on glomerular basement membrane damage increased. |
PDF Full Text Request