Study On Extracting And Separating Process Of Hederacoside C

As a kind of medicinal plants, Hedera.helix have many medicinal properties.The most important functional components were saponins,which have lots of pharmacological activities,such as protecting liver,anti-tumor,regulating immune system,alleviating convulsion and relieveing pain.This paper optimized the processes of ultrasonic extraction and reflux extraction by using the central composite design,and optimized the process of purification with macroporous adsorption resin;In addition, high-speed countercurrent chromatography(HSCCC) was used for the isolation and purification of Hederacoside C monomeric;and then identified the structure of the monomeric by mass spectrometry(MS) and nuclear magnetic resonance spectrum(NMR).The results are as follows:1.The optimum conditions of reflux extraction process were 62.6% ethanol reflux extract at 82.3℃ for 2 times,and each time for 54.7 min. Under these conditions,the extraction rate of Hederacoside C was 3.52%.And the optimum conditions of ultrasonic extraction process were 69.4% ethanol ultrasonic extract for 2 times,each time for 43.5 min, and solid-liquid ratio was 35:1, the extraction rate of Hederacoside C was 3.71%. Conditions which optimum by Central composite design was better than single factor trial,and ultrasonic extraction process was better.2.HPD-100 was determined the best macroreticular resin for purification of saponins of Hederacoside C by Static adsorption screening test. The absorption rate was 81.9% and resolution rate was 96.7%.The optimum conditions of purification of saponins of Hederacoside C were sample volume on 6BV,elution volume on 7BV with 80% ethanol,elution velocity was1BV/h.In order to promote efficiency,cost savings and suitable for industrial production,the optimum conditions were:sample volume on 6BV,elution volume on 5BV with 75% ethanol,elution velocity was 3 BV/h.3.The solvent systems of isolation and purification of Hederacoside C monomeric by HSCCC were ethyl acetate:n-butanol:water:methanol (4:3:7:1).And the structure of Hederacoside C monomeric identified by HSCCC,MS and NMR was characterized as 3-O-α-L-rhamnopyranosyl-(1â†'2)-O-a-L-arabinopyranoside (a-hederin) and its 28-O-α-L- rhamnopyranosyl-(1â†'4)-O-P-D-glucopyranosyl-(1â†'6)-O-β-D-glucopyranosyl ether.