Research Of The Mechanism Of Wnt-1 Induced Secreted Protein 1, WISP-1, Promoting Wound Healing In Human Fibroblast Cell CCD966SK

WISP-1 (Wnt-induced secreted protein-1), from the CCN gene family, also call CCN4, which is involved in many cellular activities such as growth, differentiation, cell motility, adhesion and division. Previously study display WISP-1 can induces cell adhesion and cell motility via cellular surface integrin. It also promotes osteosarcoma cells migration and metastasis via expression of MMPs. However, the effect of WISP-1 on wound healing activity in human fibroblasts cells is mostly unknown. Here, we found that WISP-1 increased the wound healing and expression of matrix metalloproteinase (MMP)-2 in human fibroblasts cells (CCD966SK cells). Transfection of cells with MMP-2 siRNA reduced WISP-1-mediated wound healing and MMP-2 expression. In addition, transfection of cells with miRNA-519d mimic also reduced WISP-1-mediated wound healing and MMP-2 expression. Taken together, our results suggest that WISP-1 down-regulating miR-519d then increases MMP-2 expression and subsequently promoting wound healing in human fibroblasts cells.