The Effect Of SPC On MMP-1,MMP-2,MMP-3 Expression In Human Dermal Fibroblasts

Objective: The purpose of this study was to investigate the effect of SPC on MMP-1,MMP-2,MMP-3 expression in human dermal fibrolast cells, and discuss the role of SPC accelerated skin wound healing process.Methods: Human demal fibroblast cells were cultivate from foreskin digested with DispaseⅡ. Cells between the fourth and tenth passages were used in this study. The reverse transcription and polymerase chain reaction (RT-PCR) method was applied to examine the production of the MMP-1,MMP-2,MMP-3 mRNA by demal fibrblast cells with SPC. Western blotting analysis was investigated the expression of the SPC-induced MMPs in human demal fibroblast cells in course of culture (2 to 48 hours).Results: Human demal fibroblast cells were successfully cultivated from foreskin digested with DispaseⅡ.The results of RT-PCR method revealed that SPC could increase the level of MMP-1,MMP-3 mRNA expression. The level begin to increase on hour 2 and persist throughout the entire culture period. But the increase of MMP-2 is insignificant. Western blotting analysis showed that the SPC-induced MMP-1,MMP-3 increased effect displayed time-dependent manner.Conclusion: In summary, these results suggest that SPC could have the potential to stimulate the production of elevated levels of MMP-1,MMP-3 in human demal fibroblast cells and balance ECM. Maybe one of the mechanisms that SPC improve skin wound healing.