Study The Therapy Of Human Umbilical Cord Mesenchymal Stem Cell On Diabetic Peripheral Neuropathy

ObjectiveTo observe the therapeutic effect of human umbilical cord mesenchymal stem cells(HUC-MSCs) transplantation on patients with diabetic peripheral neuropathy（DPN）.MethodsThe study was divided into two parts. Firstly, the umbilical cord from full-termneonates delivered abdominally were collected in sterile condition. HUC-MSCs wereobtained byexplant method, and then culture and detect it. Secondly, FBG，PBG，HbA1C levels，Toronto clinical scoring system（TCSS），and electrophysiologicalexamination were measured in32patients with diabetic peripheral neuropathy beforeand1,3,6months after they underwent HUC-MSCs transplantation. Observe thechanges of clinical symptoms and the adverse reactions.Results1.Mesenchymal stem cells derived from human umbilical cord were spindle-shapedadherent cells. They have strongly proliferation ability. Flow cytometry analysis showedthat HUC-MSCs from the standard inbred srtains were positive for the surface antigensCD44, CD73, CD90, CD105;negative for CD34, CD45, CD19, CD11b. The rate ofpositive cells exceeded95%,which tested the purity of cells was very high.2. After transplantation6cases（18.75%）completely stop using the hypoglycemicdrugs and only through diet and exercise to adjust glucose levels;24cases（75.00%）dosage down after transplantation.;there is no change in2cases（6.25%）;no patientdosage increased.3.The three follow-up results of show that the FBG，PBG，HbA1C levels，TCSS，the distal motor latency of the median nerve，the sensory conduction velocity and thedistal motor latency of the ulnar nerve, the positive rate of sensory conduction of theposterior tibial nerve, the distal motor latency and the positive rate of the sensory conduction of the common peroneal nerve all obviously improved,P<0.05.The gross andfine motor function was most rapidly improved in1month afer the therapy and it couldbe continuously improved within6months after the therapy.4. Divided the32patients into two groups:completely stop using hypoglycemicdrugs group and continue to use hypoglycemic drugs group.The duration of diabetes，HbA1C level,compliance,the preoperative abnormal rate and the postoperativeimprovement rate of lower limb nerve sensory conduction between two groups havestatistically significant.（P<0.05）5.During the study period no serious adverse reaction occurred.ConclusionHUC-MSCs transplantation can significantly improve the glucose levels,the clinicalsymptoms and electrophysiological examination of patients with diabetic peripheralneuropathy. It is a new safe, effective method to treat diabetic peripheral neuropathy.