Chemical Constituents From The Flower Of Gardenia Jasminoides Ellis

Gardenia jasminoides Ellis, an evergreen shrub, is a plant of Gardenia in Rubiaceae, which grows in the south of the Yangtze River of China. In the Chinese Materia Medica, The fruit of G. jasminoides is belong to bitter and cold and enters the heart, lung and triple burner meridians, which can be used for the remedy for pyreticosis and vexation, the liver fire and hot eyes, headache, damp-heat jaundice, stranguria, haemorrhoids and pyogenic infections. The flower of G. jasminoides is belong to bitter and cold and enters the lung and liver, which can be used for the remedy for cough with lung heat and epistaxis. Previous phytochemical investigations on the flower of G. jasminoides have revealed the presence of iridoids, triterpenoids. However, there is shortcoming on the depth and breadth of the study of the chemical constituents of flower of G. jasminoides. Besides, there are fewer reports of pharmacological effects of the flower of G. jasminoides. In this paper, we have described phytochemical investigations of flower of G. jasminoides and analysis of fatty acids of petrodeum ether extract from the flower of G. jasminoides by GC-MS, aimed at providing a scientific basis to the further research and development and utilization of G. jasminoides.This paper mainly includes the following aspects of content.1. The chemical constituents of the flower of G. jasminoides have been isolated by a variety of means of separation and their structures were elucidated through spectroscopic techniques (NMR) and physicochemical properties.38 compounds were isolated from the flower of G. jasminoides, and among them 34 were identified, which include 15 iridoids,7 flavones,3 triterpenes,2 anthraquinone, 1 diterpenes,2 steroids,3 fatty acids,1 polyhydric alcohol. And their structures were identified as garjasmine (1), dunnisin (2), β-gardiol (3), α-gardiol (4),6’-O-trans-p-coumaroylgeniposide (5), diffusoside A (6), diffusoside B (7), geniposide (8), deacetylasperulosidic acid methyl ester (9), scandoside methyl ester (10), gardenoside (11), genipin gentiobioside (12), genameside C (13), deacetylasperulosidic acid (14), shanzhiside (15); 5-hydroxy-7,3’,4’,5’-tetramethoxyflavone (16), 5-hydroxy-6,7,3’,4’,5’-pentamethoxyflavone (17),5,2’-dyhydroxy-7,3’,4’,5’-tetramethoxyflavone (18), 5,7,3’-trihydroxy-6,4’.5’-trimethoxyflavone (19),5,7.3’,5’-tetrahydroxy-6,4’-dymethoxyflavone (20), kaempferol (21), quercetin (22); 3β,23-dihydroxy-urs-12-en-28-oic acid (23), 3β,19a-dihydroxy-urs-12-en-28-oic acid (24),3(3,19a,23-trihydroxy-urs-12-en-28-oic acid (25); emodin (26), physcion (27); crocin-I (28);β-daucosterol (29),β-sitosterol (30); stearic acid (31), palmitic acid (32), oleic acid (33); mannitol (34). Compounds 1-34 were isolated from the flower of G.jasminoides and compounds 1,2,3,4,6,7,13,15,23,24 were isolated from genus Gardenia for the first time.2. Fatty acids of petrodeum ether extract from the flower of Gardenia jasminoides Ellis were analysed by GC-MS. The results showed that the five kinds of relatively high content of fatty acids were hexadecanoic acid, (Z,Z)-9,12-octadecadiennoic acid, E-11-octadecenoic, octadecanoic, eicosanoic acid.