| Objective: Through the animal experiment to observe the bone hydrophobic particles(experience) to go to ovary osteoporosis rats bone metabolism index(bone resorption and bone formation markers) and inflammatory factor, the influence of bone hydrophobic particles on postmenopausal osteoporosis(PMPO) regulation of bone absorption and bone formation and expression of inflammatory markers intervention,to provide theoretical basis for the clinical application of traditional Chinese medicine to prevent and control PMPO.Methods:1.Experimental rat model: 90 three-month old healthy female non-pregnant SD rats of clean grade were selected and sham operation group(n=15)andrandomly divided into operation model group(n=75). Bilateral ovarians were surgically resected for animals in the operation model group to establish a PMOP model, and for the sham operation group, only a small amount of fat tissue around the ovary was surgically removed.2weeks after the operation, 2 rats were randomly selected from each of the operation model group and the sham operation group, and left hind limb femur of each animal was removed and examined by pathological tissue sections to determine the establishment of the model. Upon success of the model, rats from the operation model group were randomly divided into 5 groups: model group, estradiol valerate group,low-, moderate-, and high-dose Gushu granules groups, with 10 rats in each group.2.Grouping and dose: Blank control group, model group was given saline 10 ml/kg.(d)to fill the stomach; Pentanoic acid estradiol group was given pentanoic acid estradiol tablets 0.09 mg/kg.(d) to fill the stomach; Bone hydrophobic particles high, medium and low dose groups, respectively in accordance with the higher dose of 5.4 g/kg, d), the dose of 2.7 g/kg.(d), low dose of 1.35 g/kg.(d) to fill the stomach. Continuous lavage 12 weeks.3.Indicators: Different drug intervention after 12 weeks in a row, put to death before the rats to collect each rat urine centrifugal waiting for inspection; Femoral artery blood centrifugal waiting for inspection.(1) blood blood calcium, phosphorus, uric calcium(Ca), urine creatinine(Cr) use of biochemical method for testing;(2) the bone formation markers in serum osteocalcin(OC), serum alkaline phosphatase(ALP) and bone resorption markers tartrate resistant acid phosphatase(TRAP), urine deoxidization pyridine moiety(DPD) and inflammatory factor, IL- 6, IL- 1 TNF alpha adopt ELISA analysis;(3) in the rat femur bone trabecular number and trabecular bone morphometry detection area percentage, separating degree, bone trabecular bone trabecular thickness.Results:(1) sampling detection: rats bone tissue morphology of bone trabecular number and trabecular bone area percentage, separating degree, bone trabecular bone trabecular thickness 4 indicators between the control and operation for module between the difference was statistically significant(P < 0.01).(2) the serum levels of minerals,phosphorus and calcium: pentanoic acid estradiol group, bone and blood phosphorus concentration of hydrophobic particles high dose group was obviously higher than that of model group(P < 0.01), bone and blood phosphorus levels hydrophobic particles high dose group was obviously higher than that of low, middle dose group(P< 0.01), bone hydrophobic particles high-dose group of blood calcium concentration with pentanoic acid estradiol group no difference(P > 0.05), bone hydrophobic particles low blood calcium concentration significantly lower than the high dose group, middle dose group(P < 0.01).(3) the bone formation markers(OC, ALP), bone hydrophobic particles high-dose group OC higher than that of valeric acid estradiol group(P < 0.05), bone hydrophobic particles OC is lower than the high dose group,low dose group(P < 0.05); Bone hydrophobic particles high-dose group concentration of ALP and pentanoic acid estradiol group no difference(P > 0.05), bone hydrophobic particles ALP concentration is lower than the high dose group, low dose group(P <0.05).(4) the bone resorption markers(TRACP, DPD, Ca/Cr) : bone hydrophobic particles between the high dose group and pentanoic acid estradiol way TRACP no difference(P > 0.05), bone hydrophobic particles high TRACP concentration is lower than the low dose group, middle dose group(P < 0.05); Bone hydrophobic particles of different dose groups with DPD pentanoic acid estradiol group were statistically significant(P < 0.05), bone hydrophobic particles high DPD concentration is lower than the low dose group, middle dose group(P < 0.05); Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way Ca/Cr) no difference(P > 0.05), high dose group of lower levels of Ca/Cr is better than that of low, middle dose group(P < 0.05).(5) inflammatory cytokines(IL- 1, IL- 6, TNF alpha) : bone hydrophobic particles between the high dose group and pentanoic acid estradiol way IL- 1 no difference(P > 0.05), lower levels of L- 1 high dose group is superior to the low, middle dose group(P < 0.05); Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way IL- 6 no difference(P > 0.05), high dose group of lower levels of L- 6 is better than that of low, middle dose group(P < 0.05);Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way TNF alpha difference was statistically significant(P <0.05), bone hydrophobic particles comparison between different dose groups, high, medium and high dose and low dose of no difference between the way(P > 0.05).(6) bone tissue morphology detection: bone hydrophobic particles between the high dose group and pentanoic acid estradiol way bone trabecular number no difference(P > 0.05), the number increased bone trabecular bone hydrophobic particles high-dose group is better than that of low,middle dose group(P < 0.05); Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way percentage of trabecular bone area of no difference(P > 0.05), increase percentage of trabecular bone area of high dose group is superior to the low, middle dose group(P < 0.05); Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way bone trabecular separation degree no difference(P > 0.05), high dose group of bone trabecular separation degree is lower than the lower, middle dose group(P < 0.05); Bone hydrophobic particles between the high dose group and pentanoic acid estradiol way bone trabecular thickness without difference(P > 0.05), high dose group of bone trabecular thickness is higher than the low, middle dose group(P < 0.05).Conclusions: 1, the postmenopausal osteoporosis rats model to copy success; 2, the use of bone hydrophobic particles with pentanoic acid estradiol blood phosphorus levels after treatment, blood calcium level drops, speculated that hydrophobic particles can effectively inhibit bone absorption, thus inhibiting bone calcium into the blood; 3, the use of bone hydrophobic particles with pentanoic acid estradiol therapy in rats after OC, ALP were reduced, shows that hydrophobic particles can effectively inhibit postmenopausal bone in rats bone high transition state; 4, both drugs can reduce TRACP, DPD, Ca/Cr, speculated that bone hydrophobic particles can inhibit bone absorption. 5, both drugs can inhibit, IL- 6, IL- 1 the expression of TNF alpha,speculated that influence bone hydrophobic particles can inhibit inflammation process of osteoporosis; 6, both drugs can increase osteoporosis model rats bone trabecular number and trabecular bone area percentage and bone trabecular thickness, decrease the thickness of the bone trabecular, speculated that the role of hydrophobic particles can indirectly promote bone formation. The results show that the bone hydrophobic particles by regulating bone metabolism, affect bone inflammation, have treatment effect on postmenopausal osteoporosis and its curative effect with pentanoic acid estradiol. |
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