The Role Of Glutathione In Live Oral Squamous Cell Carcinoma Cell Cal-27 Cells’ Process Of Chemotherapeutic Resistance

Background:Oral squamous cell carcinoma (oral squamous cell carcinoma, OSCC) is one of the most common malignant tumor in head and neck, in recent years the incidence increased. With the continuous improvement of surgiCal techniques and adjuvant therapy has been continuously strengthened, the overall treatment effect of OSCC has made great progress in the past few years. But for advanced cancer, although adding adjuvant radiotherapy means of induction chemotherapy before surgery and after surgery, is still difficult to achieve higher survival rate in five years. The rate of survival has become a hot research topic and seek early diagnosis and treatment of new methods to improve the OSCC patients after operation.The tumor cells to apoptosis induced cell apoptosis escape resistance is one of the main reasons of the occurrence of tumor drug resistance. Induction of apoptosis is one of the main antitumor mechanism of most anticancer drugs. Active oxygen free radical (ROS), including O2-, H2O2 and H02, OH, etc., are generated in the normal metabolic processes in living organisms. The excessive accumulation of ROS can cause cell damage, such as lipid peroxidation, protein oxidation, enzyme inactivation in vivo, DNA oxidative damage. The main mechanism of many chemotherapeutic drugs induced apoptosis of tumor cells is the overproduction of ROS in tumor cells. Therefore, ROS mediated tumor cell apoptosis effect has become a research hotspot.Cells in order to protect against ROS damage to cells and in cells exist two redox system, non enzyme system anti peroxide and enzyme antioxidant. The former includes reduced sulfur oxygen and glutathione (glutathiose GSH) protein CTRX), the latter including superoxide dismutase, catalase, glutathione peroxidase (GPX). They can make clear ROS, intracellular ROS at a relatively stable level, in order to protect cells from damage. The glutathione (GSH) as the center of the oxidation reduction system is the most important system to prevent ROS damage in cells, GSH three is a peptide containing y- amide bond and mercapto, composed of glutamic acid, cysteine and glycine. Widely existing in mammalian cells. When cells experience oxidative stress generated a lot of H2O2, GSH in the role of glutathione peroxidase, the reduction of H2O2 into H2O, its are oxidized to oxidized glutathione (GSSG), GSSG in glutathione reductase and NADPH by oxygen, reduced to GSH, body of free radiCal scavenging reaction can continue. So GSH in tumor cells to chemotherapeutic drugs play an important role in resistance.Objective:Application of laser scanning confoCal microscopy, at molecular and cellular levels of GSH represent resistance to ROS system the chemotherapy resistance role, screening the high sensitivity of chemotherapy and through the regulation of GSH level has reached the best effect of chemotherapy.Methods:We first we Cal-27 to import the GSH fluorescent probe squamous cell carcinoma cell line, detect static content and distribution of GSH Cal-27 cells in squamous cell carcinoma cell line. Then, the cliniCal concentration and ratio of head and neck cancer most commonly used three chemotherapy cisplatin, cisplatin plus 5-flouruorail, cisplatin+5FU+paclitaxel to processing squamous cell carcinoma cell line Cal-27, changes in the content of GSH in the cells were observed under laser confoCal microscopy, derived GSH curve with the change in the concentration of time to evaluation of GSH in resisting effect of different chemotherapy drugs.Results:1. GSH in Cal-27 cells distributed mainly in mitochondria, while the distribution in the cytoplasm also have a certain amount of.2. Cells Cal-27 after exposure to chemotherapeutic drugs produced rapid shrinkage process, followed by a slow relaxation, but the final volume is still less than the initial volume. This process continued for about 2 minutes.2 minutes after the cells began a slow process of shrinkage, and cells to produce new GSH, about 12 minutes after the intracellular GSH level reached a new balance.3.5FU+cisplatin paclitaxel chemotherapy with cisplatin and cisplatin+5FU chemotherapy can effectively reduce the level of GSH cells more effectively kill Cal-27 cells.Conclusion:GSH fluorescent probe can be real-time detection of GSH concentration in Cal-27 cells.+5FU+cisplatin paclitaxel can significantly reduce the level of GSH in the cell.