| As the development of economy and the improvement of living standard in China, there is a huge change going on with people’s daily diet, including an increase intake of fat. Meanwhile, people tend to takeless exercise due to a faster life pace, which leads to a fat accumulation, especially the amount of hepatic fat, and it will eventually cause fatty livers and the hepatic apoptosis. Chlorogenic acid, known as a natural product, exists in most of the food, which can regulate blood sugar, lower blood lipids and oxidative stress etc. However, no research has been done on how chlorogenic acid influence apoptosis of liver cells which caused by lipid disorders.This study mainly focused on the effect of chlorogenic acid on the related gene expressions of nonalcoholic fatty liver apoptotic cells in the SD rats which fed with high fat fodder, and also discussed the mechanism of treatment to liver apoptosis by chlorogenic acid.40 male SD rats (SPF level and weigh 210-230 g)were used in the experiment. The male SD rats were divided into 4 groups, labeled as control group (NC), high fat feeding group (HFD) and high fat feeding with low and high doses of chlorogenic acid groups (20mg·kg-1·d-1.90mg·kg-1·d-1,HFD-LC、HFD-HC), with 10 in each group. Group NC was fed with normal fodder and the rest of the groups were fed with high fat fodder. Each group was weighed daily regularly, and all the rats were executed 12 days since the beginning of the feeding. Pathological conditions have been observed by hematoxylin and eosin staining. We measured liver weight and liver index. We also retrieved serum to detect the contents of alanine aminotransferase (ALT), free fatty acids (FFA), total cholesterol (TC), triglycerides (TG), Low-density lipoprotein cholesterol (LDL-c), and High-density lipoprotein cholesterol (HDL-c), measured the expression levels of liver CD95 receptor molecule (Fas), CD95 ligand molecule (FasL), tumor necrosis factor-a (TNF-a), tumor necrosis factor receptor 1 (TNFR-1), Caspase-8, Caspase-3, B- cell lymphoma/leukemia -2 proto-oncogene (Bcl-2), Bcl-2-related genes (Bax), Nuclear transcription factor-κB (NF-κB), Interleukin-6 (IL-6), apoptotic function of p53 stimulated protein (p53) mRNA, by using semi-quantitative RT-PCR.Compared to the rats from NC group, SD rats in group HFD showed an increase in weights, liver weight and liver index, sections indicated a large amount of fat vacuoles inside the cells of the rats in HFD group along with apoptotic morphology, as well as the expression of apoptosis inducing factor increased and apoptosis inhibitors decreased. After chlorogenic acid intervention, weight, liver weight and liver index of the SD rats decreased; the number of fat vacuoles decreased and improved cells physiological state; apoptosis inducing factor expressions decreased and apoptosis inhibitors expressions increased. High dosage of chlorogenic acid led to more significant effect among all results.In conclusion, long-term high-fat diet will lead to metabolism disorders, fatty liver, cause oxidative stress and liver apoptosis. While chlorogenic acid can improve lipid metabolism, reduce the incidence of apoptosis caused by high-fat diet, and protect liver; a larger dosage of chlorogenic acid has more significant effect. |
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