Clinical Application Of The Platelet-rich Fibrin Assisted Autologous Fat Tissue Transplantation

Objective Through the establishment of animal models, to explore the Platelet-rich fibrin effects on granular fat transplantation survival rate。On the basis of the results of animal experiments studies, To apply Platelet-rich fibrin in facial granular fat transplantation on the treatment of facial atrophy so as to study the Platelet-rich fibrin auxiliary particle fat autograft in the feasibility and effectiveness of clinical application.Methods(1)Animal studies: Choose 24 male or female of Rabbits were randomly divided into two groups, each 12. Experimental group(AG + PRF) autologous adipose granule mixed platelet rich fibrin transplantation group; The control group(AG + NS) mixing saline transplantation autologous adipose granule. After the transplant survival rate were observed and histological.(2): Platelet-rich fibrin membrane VEGF release experiment: Through 10 cases of platelet- rich fibrin membrane sample for testing,study release of growth factors.(3): Platelet-rich fibrin-assisted self-clinical applications autologous adipose granule transplantation: Choose January 2013 to September 2015 in our hospital from fat particle transplantation in 61 patients. 60 cases of patients with the disease face divided into two groups,:Experimental group(AG + PRF) autologous adipose granule mixed platelet rich fibrin transplantation group; The control group Pure autologous fat particle transplantationResults Experiment I: Animal experiments: Compare the rate of fat survival : After 4w、8W、12w, Experimental group fat’ survival rate was(80.75±5.32)%,(73.50±4.04)%,(74.75±5.31)%, the survival rate of control group was(69.50±5.26)%,(60.75±5.62)%,(53±7.07)%, the difference between the two groups were statistically significant(P <0.05). Platelet-rich fibrin promote adipose tissue has obvious promoting role in survival and rebuilding. Experiment II: Platelet-rich fibrin membrane VEGF release experiment: By platelet rich fibrin membrane samples were 10 cases of VEGF release can be quantitatively detected after the release of growth factors from platelet rich fibrin successfully prepared, The fastest speed to within 4 hours and week sustained release confirmed platelet rich fibrin sustained release of vascular regeneration after tissue transplantation necessary growth factors after transplantation of adipose tissue vascularization to establish a key role. Experiment III: All cases were no obvious complications. Facial filling injections were followed up to six months after surgery, the experimental group tripartite evaluation excellent 83.3%. The control group was 56.7%; the experimental group and the control group good rate evaluation was significant difference(p <0.05). Poland syndrome Surgery achieved good results.Conclusion Platelet-rich fibrin assisted fat particle transplantation platelet rich fibrin particles can promote autologous adipose tissue graft vascularization and growth, improve the survival rate after transplantation, improved after transplantation, has broad clinical application.