The Polymorphism Of FSHβ Gene Promoter In Patients With Polycystic Ovary Syndrome

Background Polycystic ovarian syndrome (PCOS) is a common endocrine disease in women, often accompanied by persistent anovulation, insulin resistance and hyperandrogenism. Multiple factors involved in the pathogenesis of PCOS such as genetic factors and environmental factors. From our study, we found that -211 G to T mutation in the promotor of FSHP may be associated with serum free FSH. Because of the importance of FSH in the pathogenesis of ovarian dysfunction, and no further study has shown that FSHβ-211G-T mutation associated with PCOS. So in this study, we get the promoter sequence and construct the recombinant plasmid for dual-Luciferase reporter assay, and the relationship between FSHβ gene polymorphism and PCOS from FSH transcriptional regulatory was investigated.Objective To clone and analysis the activity of the FSHB promotor.Methods By using PCR, we got the promotor of FSHB from human DNA, then we inserted the amplification products into vector for sequencing. After sequencing, we put the promotor of FSHB into the pGL6 luciferase reporter vector (pGL6-TA-FSHB-promoter) for luciferase assay. The Dual-Luciferase reporter assay system was performed to analysis the activity of pGL6-TA-FSHB-promote vector.Results We obtained the promotor of FSHB by PCR. By using the Dual-Luciferase reporter assay system, we found that pGL6-FSHB-promote vector had promotor activity. After mutating FSHB-211 G to T, the luciferase expression had higher efficiency than before (p<0.05).Conclusions We cloned human FSHB gene promoter and constructed luciferase reporter vector (pGL6-FSHB-promote). This study may be helpful for further investigation about the regulation mechanism of FSHB expression. The promoter of FSHβ-211G-T may be associated with the onset of PCOS.