Study On Plasma Expression Level Of MiR-25-3p, MiR-664a-3p And MiR-744-5p And Target Gene Prediction In Systemic Lupus Erythematosus Patients

Objectives To evaluate the expression level of mi R-25-3p, mi R-664a-3p and mi R-744-5p insystemic lupus erythematosus(SLE) patients, and to investigate their correlations with clinical features and laboratory indexes. Bioinformatics methods were used to predict the target genes of mi RNA and their biological function.Methods A case-control study was adopted in this study. A total of 37 patients with SLE was recruited from the First Affiliated Hospital of Anhui Medical University and Anhui Provincial Hospital.A total of 39 controls was selected from healthy volunteers. Patients and controls were matched in terms of gender and age. All the patients with SLE were diagnosed according to the American College of Rheumatology(ACR) 1997 revised classification criteria. Three to five ml venous blood from every patient was collected and the plasma was extracted for sparing. The different expression of mi R-25-3p, mi R-664a-3p and mi R-744-5p in plasma between patients and controls was detected by real-time quantitative polymerase chain reaction(Real time PCR). Combining with demographic data and clinical index data, the association between the clinical characteristics and abnormal expression of mi RNAs in patients with SLE was explored. Finally, target gene of differentially expressed mi RNAs was predicted, and their biological functionswere analyzed using bioinformatics methods.Results(1)The plasma expression of mi R-25-3p was sigificantlyincreased in the patients with SLE compared with healthy controls(Z=-2.489,P=0.013). However, the expression of mi R-664a-3p and mi R-744-5p were not significantly different between two groups(both P>0.05).(2)The expression levelof mi R-25-3p in plasma was significantly different between patients with photaesthesia and without(Z=-2.018, P=0.044). Its association with other clinical features and laboratory parameters were not found(all P>0.05). The expression of mi R-664a-3p between patients with rashes, alopecia, and thrombocytopenia or without was significantly different as well as between the anti-SSB/Ro positive and negative(all P<0.05).There was significant difference in the expression of mi R-744-5p between patients with rash or without(Z=-2.006, P=0.045). And the expression of mi R-744-5p has significance relevance with thrombocytopenia(Z=-2.915,P=0.004). The association with other clinical features and laboratory parameters were not found(all P>0.05).(3) Target gene screening results showed that target gene predicted from all database in the mi Randa, micro Cosm and Targetscan was a total of 125. Go-network analysis showed that the main function of these target genes include c AMP metabolic process, heart contraction, spindle assembly, etc. Pathway-network analysis showed that signaling pathways which mi R-25-3p regulated included Cell cycle, c AMP signaling pathway, Glutamatergic synapse, Dilated cardiomyopathy, Retrograde endocannabinoid signaling, Proteoglycans in cancer, etc.The expression of mi R-25-3p was up-regulated in patients with SLE and its expression level was correlated with light allergy symptoms. However, the expression of mi R-664a-3p and mi R-744-5p were not significantly different between two groups. The main function of mi R-25-3p target genes include c AMP metabolism(c AMP metabolic process) and signaling pathways which mi R-25-3p regulated included Cell cycle, c AMP signaling pathway. Conclusions...