Quince Seed Dichloromethane Extract Anti-asthmatic Effect And Eosinophils Apoptosis In A Rat Model Of Allergic Asthma

Objective:based on the estabilishment of ovalbumin induced asthmarat model,This study was carried out to study the mechanisms involved in protection accorded by extract of Quince(Cydoniaoblonga Miller) seed(Qs-E) toasthmatic rat by regulation of inflammatory mediator, oxidative stress and eosinophils apoptosis Methods:Sprague-Dawley male 72 rats(200-250g) were categorized into 6 groups having twelve rats in each category, Rats were sensitized with intraperitoneal(i.p.) Ovalbumin(Ova) on days 0 and 14, followed by intranasal Ovalbumin(Ova) challenge on days 24 and 27 to generate an in vivo asthma model except normal group rats.. Animals of groups I–III were vehicle control, model control(OVA) and reference control(OVA +dexamethasone, 2.5mg/kg), groups I–III were three Qs-E +Ova(0.2g,0.4g/kg and 0.8g/kg) treated groups respectively.Lung sections were analyzed histologically,the bronchoalveolar lavage fluid(BALF) cellular differential and the concentrations of IL-4, IL-5, LTB4,and total IgE,OVA specificIgE, MDA, NO,SOD and GSH, in lung tissue homogenates were measured. the immunohistochemistry S-P method was done for measure expression of COX-2 and 5-LOX in the lungas well asApoptosis was detected by TUNELand the expressionsofFasmRNA andBcl-2mRNA in the lung tissue were detected in Situhybridization. Results:1.compared to the normal group,model control OVA group showed a massive infiltration of the peribronchial regions of the lung with inflammatory cells mainly eosinophils,this inflammation resulted in the narrowing of the bronchi.,in the peribronchial regions in asthmatic rats.compered to the model control group,Qs-E significantly reduced the number of inflammatory cells in BALF and lung tissues, decreased IL-4, IL-5, LTB4 total IgE,OVA specificIgE,MDA and NOlevel in lung, and increased GSH and SOD activity.There were a marked suppression of COX-2 and 5-LOX expression in the lung,eosinophil apoptosisand the expression of FasmRNAinthe rat tissue were increased Significantly,while the expression Bcl-2mRNA decreased Significantly.Our results suggest that Qs-E may have an anti-inflammatory and antioxidant effect,promote eosinophilapoptosis,FasmRNA expression inhibit Bcl-2mRNA expressionon onallergic bronchial asthma. Conclusions: These resultsrevealed that Qs-E possesses a deepsuppressive effect on airway inflammation and oxidative stress.Further studiespoint to the need for the evaluationof the efficacyof Qs-E in the management of asthma.