Tudy On Screening Anti-Fatigue Part Of OKRA And Its Biochemical Mechanism In Mice

Nowadays, the pase of our lives are more and more faster, and fatigue has become a threat to our health. Therefore, delaying the occurrence of fatigue and promote the recovery of fatigue have become a research hotspot in nutrition, medicine and other disciplines.Okra(Abelmoschus esculentus Linn.), belonging to the family Malvaceae, is one of the most important vegetables widely grown in the world as a tonic and functional food. Studies have shown the anti-fatigue effect of okra in mice. This study established a high-intensity induced physical fatigue animal behavior model. The objective of the study is to investigate the anti-fatigue part of okra tender fruit. The antioxidant capacity of liver and the degree of mobilization of fat would be analysis to discuss the anti-fatigue mechanism of okra.First, the speed of treadmill was adjusted to explore the exhaustive time in mice, and 18m/min was chosen as the modeling parameter. The treadmill model was used to investigate the anti-fatigue effect of okra water extracts(4,8g/Kg) in mice. The exhaustive time of treadmill and weight-loaded swimming were respectively measured, then all mice were sacrificed, blood urea nitrogen(BUN), blood lactic acid(LD), liver glycogen(LG) levels were measured in serum and liver. The results shows that feeding dietary high-dose okra extracts to mice could significantly prolong the exhaustive running time and swimming time compared with normal control group (P<0.01). Furthermore, dietary okra extracts decreased the levels of BUN(P<0.01) and LD(P<0.001) levels, but had no obvious influence on LG levels.On this basis, the parameter of treadmill was optimized and 22m/min was chosen as the exhaustive treadmill speed. According to the results of the qualitative reaction of flavonoids, the okra would be divided into peel and seed. The anti-fatigue effect of the peel and seed water extracts(4,8g/Kg) in mice and the biochemical mechanisms were investigated in the study. The results shows that dietary okra seed extracts(SE) could significantly prolong the exhaustive running time(P<0.001), increase the activity of GSH-Px, T-SOD(P<0.001) in liver; decrease the level of MDA(P<0.01), the activity of GOT(P<0.01), GPT(P<0.05) and HL(P<0.05) in liver; increase the level of FFA(P<0.05) in serum. Meanwhile, the SE groups could significantly decrease the LG level(P<0.001) in liver, compared with the model exercise group and normal control group.The study indicate that high-dose okra water extracts and the okra seed water extracts can significantly enhance the endurance capacity of mice, decrease the metabolites in fatigued mice, enhance liver antioxidant capacity and fat mobilization, alleviate exhaustive fatigue-induced liver cell damage, indicating that okra extracts process an anti-fatigue effect. The open field test indicated that the anti-fatigue effect of okra extracts is not caused by the excitation of central nerve.