| The main causes of Vascular Dementia is brain damage caused by a series of cerebral vascular diseases such as stroke, brain regions of low perfusion, high blood pressure, learning memory impairment and cognitive dysfunction, meanwhile patients gradually lose their self-care ability and some patients appear blindness, constipation and other complications, the torture that not only patients suffering from disease, and the family also is paid a lot of effort. Therefore, many medical workers are worn to seek effective drugs to treat VD.XSXMN is made of the new kind of compound preparations with a variety of valuable Chinese herbal medicine, according to the theory of traditional Chinese medicine.With aromatic begin to understand, scattered, and the effect of yiqi huoxue stasis pain. Main clinical application in cerebral infarction, coronary heart disease, the treatment of patients with blood stasis syndrome, etc. A large number of studies have shown that XSXMN can reduce MDA content in rats tissue, reduce free radical oxidation of damage brain tissue, protect myocardial ischemia, improve microcirculation, inhibit thrombosis, etc, and elevated MDA, free radical damage, myocardial ischemia and is closely related to the pathogenesis of vascular dementia. Earlier stage work of our research group found that XSXMN of bilateral common carotid artery block rat vascular dementia model has a therapeutic effect on VD rats by bilateral common carotid artery ligation.Bilateral carotid artery occlusion and reperfusion, and intraperitoneal injection of sodium nitroprusside method to establish the rat VD model, after the success of modeling Wistar VD rats were divided into 5 groups: sham-operated group, model group, posit Ive group( Piracetam 0.5 g/kg),XSXMN small dose group(1.1 g/kg) and XSXMN large dose group(2.2 g/kg). The rats in XSXMN groups were treated with XSXMN for 2 months by oral administration. After Morris water maze test and pathology test, detect acetylcholine, cholinesterase, free radicals and related enzymes((MDA、SOD、CAT、GSH-Px、Na+-K+-ATPase、Ca2+-Mg2+-ATPase)in brain tissue.Behavioral ExperimentIn the Morris water maze test, compared with model group, the latency of rats in small dose group(1.1g/kg) decreased on the 3rd 、4th and 6th day(P<0.05),and the distance shortened on the 3rd and 6th day(P<0.05 or P<0.01). The latency of rats in large dose group(2.2g/kg) decreased on the 1st、2nd、3rd 、4th and 6th day(P<0.05 or P<0.01), and the distance shortened on the2nd、 3rd and 6th day(P<0.05).On the 7th day, compared with the model group, the times of crossing the platform、time in the platform and the ratio of residence time in the platform area/total time increase in small and large dose groups in 2mins(P<0.05 or P<0.01);In small dose group, in the effective distance 、residence time in the platform area、the ratio of distance in the effective area / total distance 、the ratio of residence time in the effective area/total time increased(P<0.05 or P<0.01);In large dose group, distance in the platform area and the ratio of distance in the platform area / total distance increased(P<0.05).Acetylcholine, CholinesteraseCompared with the model, the brain acetylcholinesterase(ACh E) activity and acetylcholine(ACh) content of XSXMN small dose group(1.1g/kg) has no obvious change;Acetylcholinesterase(ACh E) activity has declined, acetylcholine content increased in XSXMN large dose group(2.2g/kg).Free radicals and related enzymes in brain tissueCompared with the model, the SOD and MDA of XSXMN small dose group(1.1g/kg) have no obvious change. SOD activity increased and the MDA content reduced in XSXMN large dose group(2.2g/kg). CAT、GSH-Px、Na+-K+-ATPase and Ca2+-Mg2+-ATPase had no obvious change in XSXMN group(1.1g/kg,2.2g/kg)Pathological ExaminationIn the pathology test, the results show that XSXMN can reverse the damage of VD rats’ cerebral cortex and hippocampus.All above, XSXMN has therapeutic effect on VD rats by bilateral carotid artery occlusion and reperfusion. It can improve the learning and memory ability; It can improve the damaged cerebral cortex tissue and hippocampus; It can improve enzyme activity of resistance to free radicals related. It can reduce free radical damage and improve brain choline system function. |
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