Research On The Antitumor Effect Of Polyoxometalates On Human Breast Cancer MCF-7 Cells

Objective:Cancer is one of the major reasons of death, due to the rapid develop pace and no effective treatments. ?China Cancer Statistics 2015? in A Cancer Journal for Clinicians magazine have reported that the forecasts of the cancer incidence, mortality and survival rate. Women common cancers were breast cancer, lung cancer, gastric cancer, colorectal cancer and esophageal cancer. And the breast cancer was considered as a women killer. In all cancer types, the breast cancer was the most common type in female patients. Furthermore, the breast cancer is the first leading cause of deaths in women and fifth in all. There is an urgent need to find effective drugs to treat breast cancer and cervical cancer. Polyoxometalates were received widely attention because of their versatile structures and various applications in chemistry, materials science, catalysis, redox, magnetism and medicine. And the previous researches have shown that polyoxometalates performed potential applications in anti-bacterial, anti-viral and anti-tumor activities. Methods:Herein, a sort of polyoxometalates was prepared. The antitumor activities against MCF-7 cells the polyoxometalates were investigated in vitro using the MTT assay. The calf thymus DNA(ct DNA) binding effect of the polyoxometalates were also investigated using UV-vis absorption spectra, fluorescence quenching. In addition, the interaction between the polyoxometalates and bovine serum albumin(BSA) was evaluated by spectroscopic methods. Morphological changes and the apoptosis of tumoral cells were detected by scanning electron microscope and confocal laser scanning microscope. The changes of cell cycle were evaluated by the flow cytometry.Results:The results of the MTT indicated that polyoxometalates possessed strong anticancer activities against MCF-7 cells. The IC50 value of PW9V3, PMo10V2 and V18O42 against MCF-7 was 70.80, 61.65, 11.95 μM for 48 h. The cell cycle results showed that PW9V3, PMo10V2 and V18O42 can alter the cell cycle, leading to increased number of cells in G2/M and S phase and reduce the number of G1-phase cells. There was a characteristic absorption peak of PW9V3, PMo10V2 and V18O42 at 206 nm. Upon the addition of ct DNA solution a hypochromism effect without any red or blue shift was observed. The results indicated the interaction between PW9V3, PMo10V2 and V18O42 and ct DNA refused intercalation interaction, which was suggested the outside stacking binding. Conclusion:These results showed that PW9V3, PMo10V2 and V18O42 as antitumor agents possess high efficacy on MCF-7 cells.